Anti-Twinkle antibody (ab187517)
Key features and details
- Rabbit polyclonal to Twinkle
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Twinkle antibody -
Description
Rabbit polyclonal to Twinkle -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Twinkle aa 384-684 (C terminal).
Database link: Q96RR1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/2000. Predicted molecular weight: 77 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 77 kDa. |
Target
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Function
Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals. -
Tissue specificity
High relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43. -
Involvement in disease
Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 3
Sensory ataxic neuropathy dysarthria and ophthalmoparesis
Mitochondrial DNA depletion syndrome 7 -
Sequence similarities
Contains 1 SF4 helicase domain. -
Cellular localization
Mitochondrion matrix > mitochondrion nucleoid. Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression. - Information by UniProt
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Database links
- Entrez Gene: 56652 Human
- Entrez Gene: 226153 Mouse
- Entrez Gene: 309441 Rat
- Omim: 606075 Human
- SwissProt: Q96RR1 Human
- SwissProt: Q8CIW5 Mouse
- Unigene: 22678 Human
- Unigene: 105585 Mouse
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Alternative names
- Ataxin 8 antibody
- Ataxin8 antibody
- ATXN 8 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab187517 has been referenced in 2 publications.
- Key J et al. CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2. Cells 12:N/A (2022). PubMed: 36611846
- Torregrosa-Muñumer R et al. PrimPol is required for replication reinitiation after mtDNA damage. Proc Natl Acad Sci U S A 114:11398-11403 (2017). PubMed: 29073063