Overview

  • Product name

  • Description

    Rabbit polyclonal to Twinkle
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Twinkle aa 384-684 (C terminal).
    Database link: Q96RR1

  • Positive control

    • HL60, K562, BXPC-3, MCF7, H460 and Mouse uterus extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab187517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 77 kDa.
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.
  • Tissue specificity

    High relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43.
  • Involvement in disease

    Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 3
    Sensory ataxic neuropathy dysarthria and ophthalmoparesis
    Mitochondrial DNA depletion syndrome 7
  • Sequence similarities

    Contains 1 SF4 helicase domain.
  • Cellular localization

    Mitochondrion matrix > mitochondrion nucleoid. Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ataxin 8 antibody
    • Ataxin8 antibody
    • ATXN 8 antibody
    • ATXN8 antibody
    • C10 orf2 antibody
    • C10orf 2 antibody
    • C10orf2 antibody
    • Chromosome 10 open reading frame 2 antibody
    • IOSCA antibody
    • mitochondrial antibody
    • MTDPS7 antibody
    • PEO 1 antibody
    • PEO antibody
    • PEO1 antibody
    • PEO1_HUMAN antibody
    • PEOA3 antibody
    • Progressive external ophthalmoplegia 1 protein antibody
    • SANDO antibody
    • SCA 8 antibody
    • SCA8 antibody
    • T7 gp4 like protein with intramitochondrial nucleoid localization antibody
    • T7 gp4-like protein with intramitochondrial nucleoid localization antibody
    • T7 helicase-related protein with intramitochondrial nucleoid localization antibody
    • T7 like mitochondrial DNA helicase antibody
    • T7-like mitochondrial DNA helicase antibody
    • Twinkle protein antibody
    • Twinkle protein, mitochondrial antibody
    • TWINL antibody
    see all

Images

  • All lanes : Anti-Twinkle antibody (ab187517) at 1/500 dilution

    Lane 1 : HL60 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : BXPC-3 cell extract
    Lane 4 : MCF7 cell extract
    Lane 5 : H460 cell extract
    Lane 6 : Mouse uterus extract

    Predicted band size: 77 kDa

References

ab187517 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187517.
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