Anti-Twist antibody [10E4E6] (ab175430)
Key features and details
- Mouse monoclonal [10E4E6] to Twist
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Twist antibody [10E4E6]
See all Twist primary antibodies -
Description
Mouse monoclonal [10E4E6] to Twist -
Host species
Mouse -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB MouseHumanRecombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
Sequence:PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR
Database link: Q15672 -
Positive control
- Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabiliser. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
10E4E6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
|
ICC/IF |
Human
|
IHC-P |
Human
|
WB |
Mouse
Human
Recombinant fragment
|
All applications |
Chimpanzee
Gorilla
Common marmoset
|
Application | Abreviews | Notes |
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/200 - 1/1000.
|
|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 21 kDa.
Abcam recommends using BSA as the blocking agent. |
ICC/IF |
1/200 - 1/1000.
|
Notes |
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Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 21 kDa. Abcam recommends using BSA as the blocking agent. |
ICC/IF
1/200 - 1/1000. |
Target
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Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. -
Tissue specificity
Subset of mesodermal cells. -
Involvement in disease
Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7291 Human
- Entrez Gene: 22160 Mouse
- Omim: 601622 Human
- SwissProt: Q8MI03 Chimpanzee
- SwissProt: Q8MIH1 Common marmoset
- SwissProt: Q8MI06 Gorilla
- SwissProt: Q15672 Human
- SwissProt: P26687 Mouse
see all -
Alternative names
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
see all
Images
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All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Raji cell lysate
Lane 6 : OCM-1 cell lysate
Predicted band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
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Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
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All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate
Predicted band size: 21 kDa -
Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein
Predicted band size: 21 kDaExpected MW is 31.9 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab175430 has been referenced in 39 publications.
- Choi IY et al. Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. Elife 9:N/A (2020). PubMed: 32011235
- Lin Y et al. Long non-coding RNA DLGAP1-AS1 facilitates tumorigenesis and epithelial-mesenchymal transition in hepatocellular carcinoma via the feedback loop of miR-26a/b-5p/IL-6/JAK2/STAT3 and Wnt/ß-catenin pathway. Cell Death Dis 11:34 (2020). PubMed: 31949128
- Dong X et al. Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis. Front Oncol 10:160 (2020). PubMed: 32154166
- Yuan Y et al. 20(S)-ginsenoside Rh2 as agent for the treatment of LMN-CRC via regulating epithelial-mesenchymal transition. Biosci Rep 40:N/A (2020). PubMed: 32141497
- Kadomoto S et al. Tumor-Associated Macrophages Induce Migration of Renal Cell Carcinoma Cells via Activation of the CCL20-CCR6 Axis. Cancers (Basel) 12:N/A (2019). PubMed: 31905918