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Anti-Twist antibody [10E4E6] (ab175430)

Reviews (3) Submit a question References (62)
Western blot - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)

Key features and details

  • Mouse monoclonal [10E4E6] to Twist
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Twist antibody [10E4E6]
    See all Twist primary antibodies

  • Description

    Mouse monoclonal [10E4E6] to Twist

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment
    Predicted to work with: Chimpanzee, Gorilla, Common marmoset

  • Immunogen

    Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
    Sequence:

    PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR


    Database link: Q15672
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.
WB (1)
1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA as the blocking agent.
ICC/IF
1/200 - 1/1000.
Notes
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA as the blocking agent.
ICC/IF
1/200 - 1/1000.

Target

  • Function

    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

  • Tissue specificity

    Subset of mesodermal cells.

  • Involvement in disease

    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS antibody
    • CRS1 antibody
    • CSO antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist family bHLH transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

Images

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : OCM-1 cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
    Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)

    Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
    Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)

    Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein

    Predicted band size: 21 kDa



    Expected MW is 31.9 kDa.

References (62)

Publishing research using ab175430? Please let us know so that we can cite the reference in this datasheet.

ab175430 has been referenced in 62 publications.

  • Pratheeshkumar P  et al. CHD4 Predicts Aggressiveness in PTC Patients and Promotes Cancer Stemness and EMT in PTC Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33419089
  • Liu G  et al. ARHGAP20 Expression Inhibited HCC Progression by Regulating the PI3K-AKT Signaling Pathway. J Hepatocell Carcinoma 8:271-284 (2021). PubMed: 33907697
  • He F  et al. ATF5 and HIF1a cooperatively activate HIF1 signaling pathway in esophageal cancer. Cell Commun Signal 19:53 (2021). PubMed: 33980247
  • Liu X  et al. Afatinib induces pro-survival autophagy and increases sensitivity to apoptosis in stem-like HNSCC cells. Cell Death Dis 12:728 (2021). PubMed: 34294686
  • Siraj AK  et al. Loss of ZNF677 Expression Is an Independent Predictor for Distant Metastasis in Middle Eastern Papillary Thyroid Carcinoma Patients. Int J Mol Sci 22:N/A (2021). PubMed: 34360599
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-Twist [10E4E6] antibody

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted May 20 2014

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