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Anti-Twist antibody [10E4E6] (ab175430)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (39)

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Western blot - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)

Key features and details

  • Mouse monoclonal [10E4E6] to Twist
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Twist antibody [10E4E6]
    See all Twist primary antibodies
  • Description

    Mouse monoclonal [10E4E6] to Twist
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
    Sequence:

    PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR


    Database link: Q15672
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabiliser.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    10E4E6
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • NIH/3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human Twist protein (ab132349)
  • Related Products

    • Recombinant Human Twist protein (ab132349)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
Recombinant fragment
All applications
Chimpanzee
Gorilla
Common marmoset
Application Abreviews Notes
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
1/200 - 1/1000.
WB (1)
1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA as the blocking agent.

ICC/IF
1/200 - 1/1000.
Notes
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA as the blocking agent.

ICC/IF
1/200 - 1/1000.

Target

  • Function

    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • Tissue specificity

    Subset of mesodermal cells.
  • Involvement in disease

    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7291 Human
    • Entrez Gene: 22160 Mouse
    • Omim: 601622 Human
    • SwissProt: Q8MI03 Chimpanzee
    • SwissProt: Q8MIH1 Common marmoset
    • SwissProt: Q8MI06 Gorilla
    • SwissProt: Q15672 Human
    • SwissProt: P26687 Mouse
    • Unigene: 66744 Human
    • Unigene: 3280 Mouse
    see all
  • Alternative names

    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS antibody
    • CRS1 antibody
    • CSO antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist family bHLH transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

Images

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : OCM-1 cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
    Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)

    Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
    Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)

    Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein

    Predicted band size: 21 kDa



    Expected MW is 31.9 kDa.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (39)

Publishing research using ab175430? Please let us know so that we can cite the reference in this datasheet.

ab175430 has been referenced in 39 publications.

  • Choi IY  et al. Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. Elife 9:N/A (2020). PubMed: 32011235
  • Lin Y  et al. Long non-coding RNA DLGAP1-AS1 facilitates tumorigenesis and epithelial-mesenchymal transition in hepatocellular carcinoma via the feedback loop of miR-26a/b-5p/IL-6/JAK2/STAT3 and Wnt/ß-catenin pathway. Cell Death Dis 11:34 (2020). PubMed: 31949128
  • Dong X  et al. Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis. Front Oncol 10:160 (2020). PubMed: 32154166
  • Yuan Y  et al. 20(S)-ginsenoside Rh2 as agent for the treatment of LMN-CRC via regulating epithelial-mesenchymal transition. Biosci Rep 40:N/A (2020). PubMed: 32141497
  • Kadomoto S  et al. Tumor-Associated Macrophages Induce Migration of Renal Cell Carcinoma Cells via Activation of the CCL20-CCR6 Axis. Cancers (Basel) 12:N/A (2019). PubMed: 31905918
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Twist antibody [10E4E6]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
human serum in 0.25% casein solution as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

Submitted Jul 07 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Twist antibody [10E4E6]

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colorectal cancer)
Permeabilization
Yes - Triton X 0.5%
Specification
Colorectal cancer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 23 2016

Western blot abreview for Anti-Twist [10E4E6] antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 20 2014

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