Name: Anti-Twist antibody [10E4E6]
SKU: ab175430
Rating: 3 (3 reviews)

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Western blot - Anti-Twist antibody [10E4E6] (ab175430)

Key features and details

Mouse monoclonal [10E4E6] to Twist
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Reacts with: Mouse, Human, Recombinant fragment
Isotype: IgG1

Product name

Anti-Twist antibody [10E4E6]
See all Twist primary antibodies
Description

Mouse monoclonal [10E4E6] to Twist
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
Sequence:
PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR

Database link: Q15672
Run BLAST with Run BLAST with
Positive control
- Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

Contains 0.5% protein stabiliser.
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

10E4E6
Isotype

IgG1
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
Recombinant Protein
- Recombinant Human Twist protein (ab132349)
Related Products
- Recombinant Human Twist protein (ab132349)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human Recombinant fragment
All applications	Chimpanzee Gorilla Common marmoset

Application	Abreviews	Notes
Flow Cyt		1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P		1/200 - 1/1000.
WB	(1)	1/500 - 1/2000. Predicted molecular weight: 21 kDa. Abcam recommends using BSA as the blocking agent.
ICC/IF		1/200 - 1/1000.

Notes
Flow Cyt 1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/200 - 1/1000.
WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa. Abcam recommends using BSA as the blocking agent.
ICC/IF 1/200 - 1/1000.

Function

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
Tissue specificity

Subset of mesodermal cells.
Involvement in disease

Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q15672 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7291 Human
- Entrez Gene: 22160 Mouse
- Omim: 601622 Human
- SwissProt: Q8MI03 Chimpanzee
- SwissProt: Q8MIH1 Common marmoset
- SwissProt: Q8MI06 Gorilla
- SwissProt: Q15672 Human
- SwissProt: P26687 Mouse
- Unigene: 66744 Human
- Unigene: 3280 Mouse
see all
Alternative names
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
- BPES2 antibody
- BPES3 antibody
- Class A basic helix-loop-helix protein 38 antibody
- CRS antibody
- CRS1 antibody
- CSO antibody
- H-twist antibody
- OTTHUMP00000116043 antibody
- SCS antibody
- Twist basic helix loop helix transcription factor 1 antibody
- Twist family bHLH transcription factor 1 antibody
- Twist homolog 1 (Drosophila) antibody
- Twist homolog 1 antibody
- TWIST homolog of drosophila antibody
- Twist related protein 1 antibody
- Twist-related protein 1 antibody
- TWIST1 antibody
- TWST1_HUMAN antibody
see all

Western blot - Anti-Twist antibody [10E4E6] (ab175430)

All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

Lane 1 : NIH/3T3 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Raji cell lysate
Lane 6 : OCM-1 cell lysate

Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)

Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)

Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.
Western blot - Anti-Twist antibody [10E4E6] (ab175430)

All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate

Predicted band size: 21 kDa
Western blot - Anti-Twist antibody [10E4E6] (ab175430)

Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein

Predicted band size: 21 kDa

Expected MW is 31.9 kDa.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab175430? Please let us know so that we can cite the reference in this datasheet.

ab175430 has been referenced in 39 publications.

Choi IY et al. Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors. Elife 9:N/A (2020). PubMed: 32011235
Lin Y et al. Long non-coding RNA DLGAP1-AS1 facilitates tumorigenesis and epithelial-mesenchymal transition in hepatocellular carcinoma via the feedback loop of miR-26a/b-5p/IL-6/JAK2/STAT3 and Wnt/ß-catenin pathway. Cell Death Dis 11:34 (2020). PubMed: 31949128
Dong X et al. Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis. Front Oncol 10:160 (2020). PubMed: 32154166
Yuan Y et al. 20(S)-ginsenoside Rh2 as agent for the treatment of LMN-CRC via regulating epithelial-mesenchymal transition. Biosci Rep 40:N/A (2020). PubMed: 32141497
Kadomoto S et al. Tumor-Associated Macrophages Induce Migration of Renal Cell Carcinoma Cells via Activation of the CCL20-CCR6 Axis. Cancers (Basel) 12:N/A (2019). PubMed: 31905918

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (skin)

Permeabilization

Specification

skin

Blocking step

human serum in 0.25% casein solution as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

Acetone

Abcam user community

Verified customer

Submitted Jul 07 2016

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Colorectal cancer)

Permeabilization

Yes - Triton X 0.5%

Specification

Colorectal cancer

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted May 23 2016

Application

Western blot

Loading amount

1e+006 cells

Gel Running Conditions

Reduced Denaturing (12%)

Sample

Human Cell lysate - whole cell (primary human macrophage)

Specification

primary human macrophage

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 20 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (39)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-Twist antibody [10E4E6]

Immunohistochemistry (Frozen sections) abreview for Anti-Twist antibody [10E4E6]

Western blot abreview for Anti-Twist [10E4E6] antibody