• Product name
  • Description
    Rabbit polyclonal to Twist
  • Host species
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide within Human Twist aa 141-190 (C terminal). The exact sequence is proprietary.


    Database link: Q15672
    (Peptide available as ab111667)

  • Positive control
    • Jurkat cell lysate.



Our Abpromise guarantee covers the use of ab49254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).Can be blocked with Human Twist peptide (ab111667).

Abcam recommends blocking with BSA.


  • Function
    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • Tissue specificity
    Subset of mesodermal cells.
  • Involvement in disease
    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS antibody
    • CRS1 antibody
    • CSO antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist family bHLH transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all


  • Anti-Twist antibody (ab49254) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000

    Predicted band size: 21 kDa
    Observed band size: 22 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry of Uterus tissue at an antibody concentration of 4-8µg/ml using anti-TWIST1 antibody (ab49254)

  • ab49254 staining Twist in mouse lung adencarcinoma induced by urethane tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 4% serum for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/100 dilution, for 16 hours at 4°C. A biotinylated goat anti-rabbit IgG polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • ab49254 staining Twist in human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

  • All lanes : Anti-Twist antibody (ab49254)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 21 kDa


This product has been referenced in:
  • Chang CH  et al. Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration, and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28718842) »
  • Zu C  et al. MiR-590-3p suppresses epithelial-mesenchymal transition in intrahepatic cholangiocarcinoma by inhibiting SIP1 expression. Oncotarget 8:34698-34708 (2017). Read more (PubMed: 28423728) »

See all 25 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

I can confirm that the recommended concentration for this product in Western Blot is 2.5ug/ml. I have also reviewed the publication listed on the datasheet and found that they had used a 2.0ug/ml concentration ...

Read More
Western blot
Mouse Cell lysate - whole cell (ES cells)
Gel Running Conditions
Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
Loading amount
18 µg
ES cells
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Maud de Dieuleveult

Verified customer

Submitted Jul 21 2017

Western blot
Mouse Cell lysate - whole cell (3T3)
Gel Running Conditions
Reduced Denaturing (12% Bis tris)
Loading amount
50 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 30 2015

IHC - Wholemount
Zebrafish Embryo (wholemount)

Abcam user community

Verified customer

Submitted Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (gel 11%)
Human Cell lysate - whole cell (ovarian cancer cell line SKOV3)
ovarian cancer cell line SKOV3
Blocking step
blocking One (Nacalai Tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C

Dr. Senn Wakahashi

Verified customer

Submitted Jul 08 2013

Thank you for your updates.

I am very sorry to hear that the customer is not satisfied with the staining.

I can certainly offer either a new vial as a free of charge replacement or a credit note which can be used for future purchase...

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Thank you for your response.

After reading through the detailed protocol you kindly forwarded to Abcam, I would make some comments:

1) Image: Could you please save the image as a jpeg file and send it back to us.

2) Antige...

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Western blot
Mouse Cell lysate - whole cell (NMuMG)
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 19 2012

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

I would be pleased to arrange a replacement product free of charge. I have added a vial of ab50887 to your next order.

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Thank you for taking time to provide that information and for contacting us with this problem. I am sorry to hear this antibody hasnotbeen providing satisfactory results.

The details provided will enable us to investigate this case and will ...

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1-10 of 19 Abreviews or Q&A


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