Product nameAnti-Twist antibody [Twist2C1a] - ChIP Grade
See all Twist primary antibodies
DescriptionMouse monoclonal [Twist2C1a] to Twist - ChIP Grade
SpecificityGood controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react. Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC.
Tested applicationsSuitable for: IP, WB, Dot blot, ChIP, ICC/IF, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment (Human)
- Purchase matching WB positive control:Recombinant Human Twist2 protein
- Recombinant Human Twist protein (ab132349) can be used as a positive control in WB. A2058 and NIH3T3 whole cell lysate.
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS
ChIP Related Products
Our Abpromise guarantee covers the use of ab50887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/50. Predicted molecular weight: 21 kDa.
Abcam recommends using BSA blocking.
|Dot blot||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
FunctionActs as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
Tissue specificitySubset of mesodermal cells.
Involvement in diseaseDefects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
All lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C
Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma
Lysates at 50 µg per lane
Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique
Blocking Step: 10% Milk for 1 hour at room temperature
Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody
ChIP analysis using ab50887 binding Twist in Human primary macrophages. Cells were cross-linked for 15 minutes with formaldehyde. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. Protein binding was detected using real-time PCR.
Ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat anti-mouse IgG at 1/200 dilution.
Lanes 2-5 : Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/50 dilution
Lane 1 : Marker lane
Lane 2 : Hela whole cell lysate, 25µg
Lane 3 : A2058 whole cell lysate, 25µg
Lane 4 : NIH3T3 whole cell lysate, 25µg
Lane 5 : F2408 whole cell lysate, 25µg
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
SDS-PAGE with 10-20% gradient gel.
ab50887 staining Twist in a Rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.
This product has been referenced in:
- de Leeuw VC et al. Look-alikes may not act alike: Gene expression regulation and cell-type-specific responses of three valproic acid analogues in the neural embryonic stem cell test (ESTn). Toxicol Lett 303:28-37 (2019). Read more (PubMed: 30578912) »
- Dou C et al. SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production. J Exp Clin Cancer Res 38:70 (2019). Read more (PubMed: 30755243) »