Anti-TWSG1/TSG antibody (ab244341)
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Overview
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Product name
Anti-TWSG1/TSG antibody
See all TWSG1/TSG primary antibodies -
Description
Rabbit polyclonal to TWSG1/TSG -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Orangutan
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Immunogen
Recombinant fragment corresponding to Human TWSG1/TSG aa 68-217.
Sequence:DECCDCVGMCNPRNYSDTPPTSKSTVEELHEPIPSLFRALTEGDTQLNWN IVSFPVAEELSHHENLVSFLETVNQPHHQNVSVPSNNVHAPYSSDKEHMC TVVYFDDCMSIHQCKISCESMGASKYRWFHNACCECIGPECIDYGSKTVK
Database link: Q9GZX9 -
Positive control
- ICC/IF: U-251 MG cells. IHC-P: Human stomach tissue.
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General notes
This product was previously labelled as TWSG1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab244341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development. -
Sequence similarities
Belongs to the twisted gastrulation protein family. -
Developmental stage
Expressed in brain throughout development. -
Domain
The N-terminal domain is sufficient to interact with BMP4. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 57045 Human
- Entrez Gene: 65960 Mouse
- Omim: 605049 Human
- SwissProt: Q9GZX9 Human
- SwissProt: Q9EP52 Mouse
- Unigene: 514685 Human
- Unigene: 10153 Mouse
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Alternative names
- PSEC0250 antibody
- twisted gastrulation homolog 1 (Drosophila) antibody
- TSG antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWSG1/TSG antibody (ab244341)Formalin-fixed, paraffin-embedded human stomach tissue stained for TWSG1/TSG with ab244341 at a 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-TWSG1/TSG antibody (ab244341)PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling TWSG1/TSG using ab244341 at 4 µg/ml (green) in ICC/IF.
Datasheets and documents
References
ab244341 has not yet been referenced specifically in any publications.
