• Product name

  • Description

    Rabbit polyclonal to TXK/RLK
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Mouse TXK/RLK aa 286-509. (Expressed in E.coli).


    Database link: P42682

  • Positive control

    • WB: Recombinant mouse TXK/RLK protein; Mouse heart tissue lysate.
  • General notes

     This product was previously labelled as TXK




Our Abpromise guarantee covers the use of ab233260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.


  • Function

    Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.
  • Tissue specificity

    Expressed in T-cells and some myeloid cell lines. Expressed in Th1/Th0 cells with IFN-gamma-producing potential.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational

    Phosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription.
    The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location.
  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. Translocates into the nucleus and enhances IFN-gamma gene transcription in T-cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • BTKL antibody
    • EC antibody
    • MGC22473 antibody
    • Protein-tyrosine kinase 4 antibody
    • PSCTK5 antibody
    • PTK4 antibody
    • Resting lymphocyte kinase antibody
    • RLK antibody
    • TKL antibody
    • TXK antibody
    • TXK tyrosine kinase antibody
    • TXK_HUMAN antibody
    • Tyrosine protein kinase TXK antibody
    • Tyrosine-protein kinase TXK antibody
    see all


  • Anti-TXK/RLK antibody (ab233260) at 2 µg/ml + Recombinant mouse TXK/RLK protein

    Predicted band size: 61 kDa

  • Anti-TXK/RLK antibody (ab233260) at 2 µg/ml + Mouse heart tissue lysate

    Predicted band size: 61 kDa


ab233260 has not yet been referenced specifically in any publications.

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