Key features and details
- Rabbit polyclonal to TXNDC13/TMX4
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TXNDC13/TMX4 antibody
See all TXNDC13/TMX4 primary antibodies
DescriptionRabbit polyclonal to TXNDC13/TMX4
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TXNDC13/TMX4 aa 299-349. The exact sequence is proprietary.
Database link: Q9H1E5
- WB: HeLa, HEK-293T, jurkat whole cell lysate. IP: HeLa whole cell lysate.
This product was previously labelled as TXNDC13
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to TXNDC13/TMX4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab242100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 μl/mg lysate
|WB||1/1000. Predicted molecular weight: 39 kDa.|
RelevanceTXNDC13 contains 1 thioredoxin domain. The exact function of TXNDC13 remains unknown.
Cellular localizationMembrane; Single pass type I membrane protein.
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All lanes : Anti-TXNDC13/TMX4 antibody (ab242100) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 10 seconds
Lysates prepared in NETN buffer.
TXNDC13/TMX4 IP in HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242100 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab242100 at 1/250 dilution.
Lane 1: ab242100 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242100 has not yet been referenced specifically in any publications.