Anti-TXNDC4 antibody (ab137611)
Key features and details
- Rabbit polyclonal to TXNDC4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TXNDC4 antibody -
Description
Rabbit polyclonal to TXNDC4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 204-406 of Human TXNDC4.
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates; SCC4 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
Target
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Function
Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1L and ERO1LB in the endoplasmic reticulum. -
Sequence similarities
Contains 1 thioredoxin domain. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 506157 Cow
- Entrez Gene: 23071 Human
- Entrez Gene: 76299 Mouse
- Entrez Gene: 100192437 Pig
- Entrez Gene: 298066 Rat
- Entrez Gene: 496539 Xenopus tropicalis
- Omim: 609170 Human
- SwissProt: Q3T0L2 Cow
see all -
Alternative names
- Endoplasmic reticulum resident protein 44 antibody
- Endoplasmic reticulum resident protein 44 kDa antibody
- Endoplasmic reticulum resident protein ERp44 antibody
see all
Images
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All lanes : Anti-TXNDC4 antibody (ab137611) at 1/1000 dilution
Lane 1 : Raji whole cell lysates
Lane 2 : H1299 whole cell lysates
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE -
ab137611, at a 1/100 dilution, staining TXNDC4 in paraffin embedded SCC4 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137611 has been referenced in 1 publication.
- Yang Y et al. Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia. Bioengineered 12:9561-9574 (2021). PubMed: 34719307