Product nameAnti-TXNDC5 antibody
See all TXNDC5 primary antibodies
DescriptionRabbit polyclonal to TXNDC5
Tested applicationsSuitable for: WB, ICC/IF, ICCmore details
Species reactivityReacts with: Mouse
His-tagged Fusion protein, corresponding to amino acids 76-417 of Mouse TXNDC5 (ERp46).
- This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2
ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.
Our Abpromise guarantee covers the use of ab10292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
FunctionPossesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.
Sequence similaritiesBelongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- EndoPDI antibody
- Endoplasmic reticulum protein ERp46 antibody
- Endoplasmic reticulum resident protein 46 antibody
ICC/IF image of ab10292 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10292 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-TXNDC5 antibody (ab10292) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 60 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This product has been referenced in:
- Alberti A et al. ERp46 is reduced by high glucose and regulates insulin content in pancreatic beta-cells. Am J Physiol Endocrinol Metab 297:E812-21 (2009). WB, ICC/IF ; Mouse . Read more (PubMed: 19622788) »
- Knoblach B et al. ERp19 and ERp46, New Members of the Thioredoxin Family of Endoplasmic Reticulum Proteins. Mol Cell Proteomics 2:1104-19 (2003). Read more (PubMed: 12930873) »