Overview

  • Product name

  • Description

    Goat polyclonal to TXNDC5
  • Host species

    Goat
  • Specificity

    This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437; NP_071368.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: SLHRFVLSQAKDEL, corresponding to C terminal amino acids 347-360 of Human TXNDC5.

  • Positive control

    • WB: HEK-293, A549, HeLa, HepG2 cell lysates. ICC/IF: HeLa and U2OS cells. IHC-P: Human Small Intestine tissue. FC: HeLa cells.
  • General notes

    GenBank Accession Number - NP_110437; NP_071368

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 23326410
Flow Cyt Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • EndoPDI antibody
    • Endoplasmic reticulum protein ERp46 antibody
    • Endoplasmic reticulum resident protein 46 antibody
    • Endothelial protein disulphide isomerase antibody
    • ER protein 46 antibody
    • ERp46 antibody
    • Hcc 2 antibody
    • MGC3178 antibody
    • PDIA15 antibody
    • Protein disulfide isomerase family A member 15 antibody
    • STRF8 antibody
    • Thioredoxin domain containing 5 (endoplasmic reticulum) antibody
    • Thioredoxin domain containing 5 antibody
    • Thioredoxin domain containing protein 5 antibody
    • Thioredoxin domain-containing protein 5 antibody
    • Thioredoxin like protein p46 antibody
    • Thioredoxin related protein antibody
    • Thioredoxin-like protein p46 antibody
    • TLP46 antibody
    • TXND5_HUMAN antibody
    • TXNDC 5 antibody
    • Txndc5 antibody
    see all

Images

  • Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

  • ab13820 (3.75 µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab13820 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

  • All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml

    Lane 1 : HEK-293 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Mouse Cell (cardiomyocyte)
Specification
cardiomyocyte
Permeabilization
Yes - 0.5% Tritonx-100
Fixative
Formaldehyde

Ms. shuang zhang

Verified customer

Submitted Dec 04 2013

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