Anti-TXNDC5 antibody (ab13820)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-TXNDC5 antibody
See all TXNDC5 primary antibodies -
Description
Goat polyclonal to TXNDC5 -
Host species
Goat -
Specificity
This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437; NP_071368. -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide: SLHRFVLSQAKDEL, corresponding to C terminal amino acids 347-360 of Human TXNDC5.
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Positive control
- WB: HEK-293, A549, HeLa, HepG2 cell lysates. ICC/IF: HeLa and U2OS cells. IHC-P: Human Small Intestine tissue. FC: HeLa cells.
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General notes
GenBank Accession Number - NP_110437; NP_071368
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab13820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). | |
| IHC-P | Use at an assay dependent concentration. PubMed: 23326410 | |
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 81567 Human
- Entrez Gene: 105245 Mouse
- SwissProt: Q8NBS9 Human
- SwissProt: Q91W90 Mouse
- Unigene: 150837 Human
- Unigene: 28622 Mouse
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Alternative names
- EndoPDI antibody
- Endoplasmic reticulum protein ERp46 antibody
- Endoplasmic reticulum resident protein 46 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (ab13820)Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC5 antibody (ab13820)ab13820 (3.75 µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Flow Cytometry - Anti-TXNDC5 antibody (ab13820)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab13820 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-TXNDC5 antibody (ab13820)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-TXNDC5 antibody (ab13820)All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang L et al. miR-573 is a negative regulator in the pathogenesis of rheumatoid arthritis. Cell Mol Immunol 13:839-849 (2016). Read more (PubMed: 26166764) »
- Wang L et al. Investigate pathogenic mechanism of TXNDC5 in rheumatoid arthritis. PLoS One 8:e53301 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23326410) »
