Recombinant
RabMAb

Recombinant Anti-TXNDC9 antibody [EPR15239] (ab191405)

Overview

  • Product name

    Anti-TXNDC9 antibody [EPR15239]
    See all TXNDC9 primary antibodies
  • Description

    Rabbit monoclonal [EPR15239] to TXNDC9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TXNDC9 aa 100-200. The exact sequence is proprietary.
    Database link: O14530

  • Positive control

    • K562, Jurkat, Raji, SW480, C6, Raw264.7 and NIH/3T3 cell lysates: Human squamous cell carcinoma of cervix tissue: SW480 cells.
  • General notes

     

     

    Previously labelled as Thioredoxin domain containing 9. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15239
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/400.

Target

  • Function

    Significantly diminishes the chaperonin TCP1 complex ATPase activity, thus negatively impacts protein folding, including that of actin or tubulin.
  • Sequence similarities

    Contains 1 thioredoxin domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • APACD antibody
    • ATP binding protein associated with cell differentiation antibody
    • ATP-binding protein associated with cell differentiation antibody
    • ES cell related protein antibody
    • PHLP3 antibody
    • Phosducin like protein 3 antibody
    • Protein 1 4 antibody
    • Protein 1-4 antibody
    • Thioredoxin domain containing 9 antibody
    • Thioredoxin domain containing protein 9 antibody
    • Thioredoxin domain-containing protein 9 antibody
    • TXND9_HUMAN antibody
    • Txndc9 antibody
    • TXNDC9 protein antibody
    see all

Images

  • Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/10000 dilution + K562 cell lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : SW80 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling TXNDC9 with ab191405 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized SW480 cells labeling TXNDC9 with ab191405 at 1/400 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

    The two negative controls used anti-TXNDC9 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

References

ab191405 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191405.
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