Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-TXNIP antibody [EPR14774] (ab188865)

Rabbit recombinant monoclonal TXNIP antibody [EPR14774]. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 5 publication(s). Independently reviewed in 4 review(s).

Overview

  • Product name

    Anti-TXNIP antibody [EPR14774]
    See all TXNIP primary antibodies
  • Description

    Rabbit monoclonal [EPR14774] to TXNIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TXNIP aa 50-150. The exact sequence is proprietary.
    Database link: Q9H3M7

  • Positive control

    • Human fetal thymus, BxPC-3, PC-12 and NIH/3T3 lysates; Human kidney and Mouse liver tissues; HeLa and U87-MG cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab188865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.
  • Sequence similarities

    Belongs to the arrestin family.
  • Post-translational
    modifications

    Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EST01027 antibody
    • HHCPA78 antibody
    • THIF antibody
    • Thioredoxin binding protein 2 antibody
    • Thioredoxin interacting protein antibody
    • Thioredoxin-binding protein 2 antibody
    • Thioredoxin-interacting protein antibody
    • TXNIP antibody
    • TXNIP_HUMAN antibody
    • Upregulated by 1,25 dihydroxyvitamin D 3 antibody
    • VDUP1 antibody
    • Vitamin D3 up-regulated protein 1 antibody
    see all

Images

  • All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TXNIP knockout HAP1 whole cell lysate
    Lane 3 : MCF7 whole cell lysate
    Lane 4 : K562 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab188865 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab188865 was shown to recognize TXNIP in wild-type HAP1 cells as signal was lost at the expected MW in TXNIP knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TXNIP knockout samples were subjected to SDS-PAGE. ab188865 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/5000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) treated with 25uM MG-132 for 4 hours whole cell lysates.
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10mM H2O2 for 1 hour whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

References

This product has been referenced in:

See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 03 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 03 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Permeabilization
Yes - 0.3% triton in PBS 10min
Specification
brain
Blocking step
10% goat serum with 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 28 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
50 µg
Specification
MDA-MB-231
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 26 2015

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