Recombinant Anti-TXNIP antibody [EPR14774] - BSA and Azide free (ab215366)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14774] to TXNIP - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TXNIP antibody [EPR14774] - BSA and Azide free
See all TXNIP primary antibodies -
Description
Rabbit monoclonal [EPR14774] to TXNIP - BSA and Azide free -
Host species
Rabbit -
Specificity
Expression levels of the target protein vary with sample type and some optimisation may be required. For western blotting, more concentrated lysates may be required when using tissues samples.
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal thymus, BxPC-3, PC-12 and NIH/3T3 lysates; Human kidney and Mouse liver tissues; HeLa and U87-MG cells. WB : Wild-type HAP1 whole cell lysate, MCF7 whole cell lysate and K562 whole cell lysate.
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General notes
ab215366 is the carrier-free version of ab188865.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14774 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. -
Sequence similarities
Belongs to the arrestin family. -
Post-translational
modificationsUbiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10628 Human
- Entrez Gene: 56338 Mouse
- Entrez Gene: 117514 Rat
- Omim: 606599 Human
- SwissProt: Q9H3M7 Human
- SwissProt: Q8BG60 Mouse
- SwissProt: Q5M7W1 Rat
- Unigene: 533977 Human
see all -
Alternative names
- EST01027 antibody
- HHCPA78 antibody
- THIF antibody
see all
Images
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All lanes : Anti-TXNIP antibody [EPR14774] (ab188865) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TXNIP knockout HAP1 whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblasts cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDaLanes 1 - 4: Merged signal (red and green). Green - ab188865 observed at 44 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab188865 was shown to recognize TXNIP in wild-type HAP1 cells as signal was lost at the expected MW in TXNIP knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TXNIP knockout samples were subjected to SDS-PAGE. ab188865 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188865).
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188865).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188865).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab215366 has been referenced in 3 publications.
- Sarina et al. Mechanism of Placenta Damage in Gestational Diabetes Mellitus by Investigating TXNIP of Patient Samples and Gene Functional Research in Cell Line. Diabetes Ther 10:2265-2288 (2019). PubMed: 31654346
- Han Y et al. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol 16:32-46 (2018). PubMed: 29475133
- Liu Y et al. TXNIP mediates NLRP3 inflammasome activation in cardiac microvascular endothelial cells as a novel mechanism in myocardial ischemia/reperfusion injury. Basic Res Cardiol 109:415 (2014). WB ; Mouse . PubMed: 25015733