Recombinant
RabMAb

Recombinant Anti-TXNIP antibody [EPR14774] - BSA and Azide free (ab215366)

Overview

  • Product name

    Anti-TXNIP antibody [EPR14774] - BSA and Azide free
    See all TXNIP primary antibodies
  • Description

    Rabbit monoclonal [EPR14774] to TXNIP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TXNIP aa 50-150. The exact sequence is proprietary.
    Database link: Q9H3M7

  • Positive control

    • Human fetal thymus, BxPC-3, PC-12 and NIH/3T3 lysates; Human kidney and Mouse liver tissues; HeLa and U87-MG cells.
  • General notes

    Ab215366 is the carrier-free version of ab188865. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab215366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab215366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.
  • Sequence similarities

    Belongs to the arrestin family.
  • Post-translational
    modifications

    Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EST01027 antibody
    • HHCPA78 antibody
    • THIF antibody
    • Thioredoxin binding protein 2 antibody
    • Thioredoxin interacting protein antibody
    • Thioredoxin-binding protein 2 antibody
    • Thioredoxin-interacting protein antibody
    • TXNIP antibody
    • TXNIP_HUMAN antibody
    • Upregulated by 1,25 dihydroxyvitamin D 3 antibody
    • VDUP1 antibody
    • Vitamin D3 up-regulated protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188865).

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TXNIP with ab188865 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188865).

References

This product has been referenced in:

  • Han Y  et al. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol 16:32-46 (2018). Read more (PubMed: 29475133) »
  • Liu Y  et al. TXNIP mediates NLRP3 inflammasome activation in cardiac microvascular endothelial cells as a novel mechanism in myocardial ischemia/reperfusion injury. Basic Res Cardiol 109:415 (2014). WB ; Mouse . Read more (PubMed: 25015733) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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