Recombinant Anti-TXNL1 antibody [EPR16061(B)] - BSA and Azide free (ab238976)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16061(B)] to TXNL1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TXNL1 antibody [EPR16061(B)] - BSA and Azide free
See all TXNL1 primary antibodies -
Description
Rabbit monoclonal [EPR16061(B)] to TXNL1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human thyroid papillary adenocarcinoma tissue. WB: HEK293T, K-562 and Caco-2 cell lysates. Flow Cyt (intra): 293 cells. ICC: HCT116 cells.
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General notes
ab238976 is the carrier-free version of ab188328.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16061(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 PITH domain.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9352 Human
- Entrez Gene: 53382 Mouse
- Entrez Gene: 140922 Rat
- Omim: 603049 Human
- SwissProt: O43396 Human
- SwissProt: Q8CDN6 Mouse
- SwissProt: Q920J4 Rat
- Unigene: 114412 Human
see all -
Alternative names
- 32 kDa thioredoxin related protein antibody
- 32 kDa thioredoxin-related protein antibody
- Thioredoxin like 1 antibody
see all
Images
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All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TXNL1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaThis data was developed using ab188328, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab188328 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.
ab188328 Anti-TXNL1 antibody [EPR16061(B)] - N-terminal was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188328).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188328).
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Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188328).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab238976 has not yet been referenced specifically in any publications.