Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

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Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Flow Cytometry (Intracellular) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Carrier Free

Overview

  • Product name

    Anti-TXNL1 antibody [EPR16061(B)] - N-terminal
    See all TXNL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Caco 2, K562, 293, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates. IHC-P: Human thyroid papillary adenocarcinoma tissue. ICC: HCT-116 cells. Flow Cyt (intra): 293 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

Target

Images

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : TXNL1 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab188328 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab188328 Anti-TXNL1 antibody [EPR16061(B)] - N-terminal was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

    Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

    Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution + 293 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution

    Lane 1 : Caco 2 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Flow Cytometry (Intracellular) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Flow Cytometry (Intracellular) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

    Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%)labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

     

     

  • Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

References (1)

Publishing research using ab188328? Please let us know so that we can cite the reference in this datasheet.

ab188328 has been referenced in 1 publication.

  • Tan SLW  et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). PubMed: 28575672

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