Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Submit a review Submit a question References (1)
Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Flow Cytometry - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
  • Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TXNL1 antibody [EPR16061(B)] - N-terminal
    See all TXNL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16061(B)] to TXNL1 - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TXNL1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O43396

  • Positive control

    • WB: Caco 2, K562, 293, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates. IHC-P: Human thyroid papillary adenocarcinoma tissue. ICC: HCT-116 cells. Flow Cyt: 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab188328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : TXNL1 knockout HEK293T cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab188328 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab188328 Anti-TXNL1 antibody [EPR16061(B)] - N-terminal was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

    Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

    Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution + 293 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution

    Lane 1 : Caco 2 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa

  • Flow Cytometry - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Flow Cytometry - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

    Flow Cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)
    Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328)

References (1)

Publishing research using ab188328? Please let us know so that we can cite the reference in this datasheet.

ab188328 has been referenced in 1 publication.

  • Tan SLW  et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell 169:1105-1118.e15 (2017). PubMed: 28575672

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab188328.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com