Product nameAnti-TXNRD1 antibody
See all TXNRD1 primary antibodies
DescriptionRabbit polyclonal to TXNRD1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human TXNRD1 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Testis and Spleen tissue lysates as well as the following whole cell lysates: HeLa; HepG2; HEK293; MCF7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab113904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%).|
FunctionIsoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Tissue specificityIsoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Contains 1 glutaredoxin domain.
DomainThe N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
modificationsThe N-terminus of isoform 5 is blocked.
Cellular localizationCytoplasm and Cytoplasm. Nucleus.
- Information by UniProt
- cytoplasmic antibody
- Gene associated with retinoic and IFN-induced mortality 12 protein antibody
- Gene associated with retinoic and interferon-induced mortality 12 protein antibody
All lanes : Anti-TXNRD1 antibody (ab113904) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 37 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The predicted molecular weight of TXNRD1 is 71 kDa (SwissProt), however we expect to observe a banding pattern around 60 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab113904 has not yet been referenced specifically in any publications.