Anti-TXNRD1 antibody (ab16840)
Key features and details
- Rabbit polyclonal to TXNRD1
- Suitable for: IP, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TXNRD1 antibody
See all TXNRD1 primary antibodies -
Description
Rabbit polyclonal to TXNRD1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Chimpanzee, Ferret, Macaque monkey, Orangutan -
Immunogen
Recombinant full length protein (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB | (2) |
1/2000. Predicted molecular weight: 55 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/2000. Predicted molecular weight: 55 kDa. |
Target
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Function
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. -
Tissue specificity
Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. -
Sequence similarities
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Contains 1 glutaredoxin domain. -
Domain
The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays. -
Post-translational
modificationsThe N-terminus of isoform 5 is blocked.
ISGylated. -
Cellular localization
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 282388 Cow
- Entrez Gene: 7296 Human
- Entrez Gene: 50493 Mouse
- Entrez Gene: 58819 Rat
- Omim: 601112 Human
- SwissProt: O62768 Cow
- SwissProt: Q16881 Human
- SwissProt: Q9JMH6 Mouse
see all -
Alternative names
- cytoplasmic antibody
- Gene associated with retinoic and IFN-induced mortality 12 protein antibody
- Gene associated with retinoic and interferon-induced mortality 12 protein antibody
see all
Images
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ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.
Lane 1: HeLa cell lysates.
Lane 2: 293T cell lysates.
Lane 3: Jurkat cell lysates.
ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.
Lane 1: HeLa cell lysates.
Lane 2: 293T cell lysates.
Lane 3: Jurkat cell lysates.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab16840 has been referenced in 24 publications.
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Wu DM et al. Nrf2 overexpression rescues the RPE in mouse models of retinitis pigmentosa. JCI Insight 6:N/A (2021). PubMed: 33491671
- Zarkada G et al. Specialized endothelial tip cells guide neuroretina vascularization and blood-retina-barrier formation. Dev Cell 56:2237-2251.e6 (2021). PubMed: 34273276
- Braunstein I et al. Opposing effects of polysulfides and thioredoxin on apoptosis through caspase persulfidation. J Biol Chem 295:3590-3600 (2020). PubMed: 32041780
- Wu Z et al. Inflammatory response and oxidative stress attenuated by sulfiredoxin-1 in neuron-like cells depends on nuclear factor erythroid-2-related factor 2. Mol Med Rep 22:4734-4742 (2020). PubMed: 33173963