• Product name

  • Description

    Rabbit polyclonal to TXNRD1
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Chimpanzee, Ferret, Macaque monkey, Orangutan
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control

    • HeLa whole cell lysate.



Our Abpromise guarantee covers the use of ab16840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Use at an assay dependent concentration.
    IHC-P: 1/400 (PMID 20064251).
    WB: 1/2000. Predicted molecular weight: 55 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
    • Tissue specificity

      Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
    • Sequence similarities

      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
      Contains 1 glutaredoxin domain.
    • Domain

      The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
    • Post-translational

      The N-terminus of isoform 5 is blocked.
    • Cellular localization

      Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • cytoplasmic antibody
      • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
      • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
      • Gene associated with retinoid IFN induced mortality 12 protein antibody
      • GRIM 12 antibody
      • GRIM-12 antibody
      • GRIM12 antibody
      • KDRF antibody
      • KM 102 derived reductase like factor antibody
      • KM-102-derived reductase-like factor antibody
      • MGC9145 antibody
      • Oxidoreductase antibody
      • Thioredoxin reductase 1 antibody
      • Thioredoxin reductase 1 cytoplasmic antibody
      • Thioredoxin reductase GRIM 12 antibody
      • Thioredoxin reductase TR1 antibody
      • TR 1 antibody
      • TR antibody
      • TR1 antibody
      • TRXR 1 antibody
      • TRXR1 antibody
      • TRXR1_HUMAN antibody
      • TXNR antibody
      • TXNRD 1 antibody
      • Txnrd1 antibody
      see all


    • ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.

      Lane 1: HeLa cell lysates.

      Lane 2: 293T cell lysates.

      Lane 3: Jurkat cell lysates.

      ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.

      Lane 1: HeLa cell lysates.

      Lane 2: 293T cell lysates.

      Lane 3: Jurkat cell lysates.

    • TXNRD1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TXNRD1 (ab16840) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16840.
      Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 55kDa: TXNRD1. Non specific band - 65kDa: We are unsure as to the identity of this extra band.
    • ab16840 at 1/100 dilution detecting TXNRD1 from human EA hy 926 cells by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected with an Alexa-Fluor 488® goat anti-rabbit IgG.

      This image is coutesy of an Abreview submitted by Adam Szadkowski on 5 January 2006.

      See Abreview

    • ab16840 staining TXNRD1 in human mammary tissue by Immunohistochemistry (paraffin embedded sections).
      Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab16840 at 1/400 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each Reagent
    • ab16840 (2µg/ml) staining TXNRD1 in human breast using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining of acini.
      Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


    This product has been referenced in:

    • Hao C  et al. MicroRNA-124 regulates the radiosensitivity of non-small cell lung cancer cells by targeting TXNRD1. Oncol Lett 13:2071-2078 (2017). WB ; Human . Read more (PubMed: 28454363) »
    • Karwaciak I  et al. TLR2 activation induces antioxidant defence in human monocyte-macrophage cell line models. Oncotarget 8:54243-54264 (2017). Read more (PubMed: 28903338) »
    See all 16 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Chinese Hamster Cell lysate - whole cell (CHO)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jan 05 2015


    Thank you for contacting us.

    As we discussed please use these antibodies without any hesitation, If these fails please let us know.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More
    Immunocytochemistry/ Immunofluorescence
    Human Cell (EA hy 926)
    EA hy 926
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1%

    Mr. Adam Szadkowski

    Verified customer

    Submitted Jan 05 2006

    Western blot
    Human Cell lysate - whole cell (EA hy926)
    EA hy926
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

    Mr. Adam Szadkowski

    Verified customer

    Submitted Nov 10 2005

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