Recombinant
RabMAb

Anti-TXNRD1 antibody [EPR10204(B)] (ab151716)

Overview

  • Product name
    Anti-TXNRD1 antibody [EPR10204(B)]
    See all TXNRD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10204(B)] to TXNRD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human TXNRD1 (UniProt: Q16881).

  • Positive control
    • Jurkat, HeLa and A549 cell lysates; Human testis tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
    • Tissue specificity
      Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
    • Sequence similarities
      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
      Contains 1 glutaredoxin domain.
    • Domain
      The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
    • Post-translational
      modifications
      The N-terminus of isoform 5 is blocked.
      ISGylated.
    • Cellular localization
      Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytoplasmic antibody
      • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
      • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
      • Gene associated with retinoid IFN induced mortality 12 protein antibody
      • GRIM 12 antibody
      • GRIM-12 antibody
      • GRIM12 antibody
      • KDRF antibody
      • KM 102 derived reductase like factor antibody
      • KM-102-derived reductase-like factor antibody
      • MGC9145 antibody
      • Oxidoreductase antibody
      • Thioredoxin reductase 1 antibody
      • Thioredoxin reductase 1 cytoplasmic antibody
      • Thioredoxin reductase GRIM 12 antibody
      • Thioredoxin reductase TR1 antibody
      • TR 1 antibody
      • TR antibody
      • TR1 antibody
      • TRXR 1 antibody
      • TRXR1 antibody
      • TRXR1_HUMAN antibody
      • TXNR antibody
      • TXNRD 1 antibody
      • Txnrd1 antibody
      see all

    Images

    • All lanes : Anti-TXNRD1 antibody [EPR10204(B)] (ab151716) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 71 kDa

    • Immunohistochemical analysis of Paraffin-embedded Human testis tissue labeling TXNRD1 with ab151716 at 1/50 dilution.

    References

    ab151716 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151716.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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