Product nameAnti-TXNRD1 antibody [EPR10205]
See all TXNRD1 primary antibodies
DescriptionRabbit monoclonal [EPR10205] to TXNRD1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Rat, Human
Synthetic peptide, corresponding to residues in Human TXNRD1 (UniProt: Q16881).
- HepG2, SH-SY5Y and HeLa cell lysates; Human pancreas tissue; HepG2 cells; permeabilized SH-SY5Y cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 71 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIsoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Tissue specificityIsoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Contains 1 glutaredoxin domain.
DomainThe N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
modificationsThe N-terminus of isoform 5 is blocked.
Cellular localizationCytoplasm and Cytoplasm. Nucleus.
- Information by UniProt
- cytoplasmic antibody
- Gene associated with retinoic and IFN-induced mortality 12 protein antibody
- Gene associated with retinoic and interferon-induced mortality 12 protein antibody
ab167411 showing +ve staining in Human normal kidney.
ab167411 showing +ve staining in Human heart.
ab167411 showing +ve staining in Human normal liver.
All lanes : Anti-TXNRD1 antibody [EPR10205] (ab167411) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 71 kDa
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TXNRD1 with ab167411 at 1/100 dilution.
Immunofluorescent staining of HepG2 cells labeling TXNRD1 with ab167411 at 1/250 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized SH-SY5Y cells labeling TXNRD1 with ab167411 at 1/100 dilution (red) or with a rabbit IgG (negative) (green).
ab167411 has not yet been referenced specifically in any publications.