Overview

  • Product name

    Anti-TYRO3 antibody [EPR4308]
    See all TYRO3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4308] to TYRO3
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TYRO3 aa 850-950. The exact sequence is proprietary.
    (Peptide available as ab218119)

  • Positive control

    • WB: Fetal brain, fetal hippocampus, MCF7, Rat hippocampus, Rat brain, Human fetal brain, and Mouse hippocampus lysates IP: SH-SY5Y whole cell lysate IHC-P: Human brain tissue, Human colon carcinoma and Human ovarian carcinoma
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 97 kDa.Can be blocked with TYRO3 peptide (ab218119).
IP 1/200.

For unpurified use at 1/10 - 1/100.

IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.
    • Tissue specificity

      Abundant in the brain and lower levels in other tissues.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Brt antibody
      • BYK antibody
      • DTK antibody
      • Etk-2 antibody
      • protein tyrosine kinase 3 antibody
      • Rek antibody
      • RSE antibody
      • SKY antibody
      • Tif antibody
      • tyro3 antibody
      • TYRO3 protein tyrosine kinase antibody
      • TYRO3_HUMAN antibody
      • Tyrosine-protein kinase byk antibody
      • Tyrosine-protein kinase DTK antibody
      • Tyrosine-protein kinase receptor TYRO3 antibody
      • Tyrosine-protein kinase RSE antibody
      • Tyrosine-protein kinase SKY antibody
      • Tyrosine-protein kinase TIF antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • All lanes : Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution (Purified)

      Lane 1 : Rat hippocampus lysates at 20 µg
      Lane 2 : Rat brain lysates at 20 µg
      Lane 3 : Human fetal brain lysates at 15 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 97 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?

    • Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution (Purified) + Mouse hippocampus lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?

    • ab109231 (purified) at 1:200 dilution (2µg) immunoprecipitating TYRO3 in SH-SY5Y whole cell lysate.
      Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab109231 & SH-SY5Y whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109231 in SH-SY5Y whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:2000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemical analysis of TYRO3 in paraffin embedded Human brain tissue, using ab109231 at a 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution

      Lane 1 : fetal brain lysates
      Lane 2 : fetal hippocampus lysates
      Lane 3 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 97 kDa

    References

    This product has been referenced in:

    See all 4 Publications for this product

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