Overview

  • Product name

    Anti-TYRO3 antibody [OTI5B7]
    See all TYRO3 primary antibodies
  • Description

    Mouse monoclonal [OTI5B7] to TYRO3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human TYRO3. Protein expressed in HEK-293T cells.
    Database link: Q06418

  • Positive control

    • ICC/IF: pCMV6-ENTRY TYRO3 cDNA transfected COS-7 cells. FC: HEK-293T cells transfected with overexpress plasmid. IP: DDK tagged TYRO3 overexpression lysates.
  • General notes

    The clone number has been updated from 5B7 to OTI5B7, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab117877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 4 µg/ml.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function

    May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity

    Abundant in the brain and lower levels in other tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Brt antibody
    • BYK antibody
    • DTK antibody
    • Etk-2 antibody
    • protein tyrosine kinase 3 antibody
    • Rek antibody
    • RSE antibody
    • SKY antibody
    • Tif antibody
    • tyro3 antibody
    • TYRO3 protein tyrosine kinase antibody
    • TYRO3_HUMAN antibody
    • Tyrosine-protein kinase byk antibody
    • Tyrosine-protein kinase DTK antibody
    • Tyrosine-protein kinase receptor TYRO3 antibody
    • Tyrosine-protein kinase RSE antibody
    • Tyrosine-protein kinase SKY antibody
    • Tyrosine-protein kinase TIF antibody
    see all

Images

  • pCMV6-ENTRY TYRO3 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for TYRO3 using ab117877 at a 1/100 dilution in ICC/IF.

  • For each experiment, 500 µl of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK-293T lysate), 2 µg of ab117877 and 20 µl (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.



    Lane 1 : Negative control: IP without adding ab117877
    Lane 2 : Anti-TYRO3 antibody [OTI5B7] (ab117877) at 2 µg

    All lanes :
  • HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY TYRO3 (Red) or empty vector control plasmid (Blue) were immunostained with ab117877 at 1/100 dilution and then analyzed by flow cytometry.

References

ab117877 has not yet been referenced specifically in any publications.

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