Key features and details
- Rabbit polyclonal to Tyrosinase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Tyrosinase antibody
See all Tyrosinase primary antibodies
DescriptionRabbit polyclonal to Tyrosinase
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human Tyrosinase (N terminal).
Database link: P14679
- HepG2 whole cell lysate (ab7900); MCF7 cells; Human stomach cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 60 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/100.|
FunctionThis is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
Involvement in diseaseDefects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
Sequence similaritiesBelongs to the tyrosinase family.
Cellular localizationMelanosome membrane.
- Information by UniProt
- ATN antibody
- CMM8 antibody
- LB24 AB antibody
Anti-Tyrosinase antibody (ab196874) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 60 kDa
Immunofluoresent analysis of MCF7 cells labeling Tyrosinase with ab196874 at 1/50 dilution. Blue: DAPI for nuclear staining.
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Tyrosinase with ab196874 at 1/100 dilution.
ab196874 has not yet been referenced specifically in any publications.