Overview

  • Product name

  • Description

    Rabbit polyclonal to Tyrosinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Tyrosinase (N terminal).
    Database link: P14679

  • Positive control

    • HepG2 whole cell lysate (ab7900); MCF7 cells; Human stomach cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab196874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/100.

Target

  • Function

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • Involvement in disease

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • Sequence similarities

    Belongs to the tyrosinase family.
  • Cellular localization

    Melanosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATN antibody
    • CMM8 antibody
    • LB24 AB antibody
    • LB24-AB antibody
    • Monophenol monooxygenase antibody
    • OCA1 antibody
    • OCA1A antibody
    • OCAIA antibody
    • Oculocutaneous albinism IA antibody
    • SHEP3 antibody
    • SK29 AB antibody
    • SK29-AB antibody
    • Tumor rejection antigen AB antibody
    • TYR antibody
    • TYRO_HUMAN antibody
    • tyrosinase (oculocutaneous albinism IA) antibody
    • Tyrosinase antibody
    see all

Images

  • Anti-Tyrosinase antibody (ab196874) at 1/500 dilution + HepG2 cell lysate

    Predicted band size: 60 kDa

  • Immunofluoresent analysis of MCF7 cells labeling Tyrosinase with ab196874 at 1/50 dilution. Blue: DAPI for nuclear staining.

  • Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Tyrosinase with ab196874 at 1/100 dilution.

References

ab196874 has not yet been referenced specifically in any publications.

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