• Product name
    Anti-Tyrosinase antibody [SPM360], prediluted
    See all Tyrosinase primary antibodies
  • Description
    Mouse monoclonal [SPM360] to Tyrosinase, prediluted
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cat, Dog
  • Immunogen

    Recombinant Human Tyrosinase

  • Epitope
    ab54447 reacts with an epitope located in the region encoded by amino acids 1-433.
  • Positive control
    • Melanoma cell lines and Melanoma tissue.



Our Abpromise guarantee covers the use of ab54447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: Ready-to-use for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
    • Involvement in disease
      Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
      Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
    • Sequence similarities
      Belongs to the tyrosinase family.
    • Cellular localization
      Melanosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all


    • Ab54447 staining human Tyrosinase in human melanoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.


    This product has been referenced in:
    • Clotet S  et al. Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease. Mol Cell Proteomics 16:368-385 (2017). Read more (PubMed: 28062795) »
    • Guan C  et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). Read more (PubMed: 29456788) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    Thank you for submitting an Abreview for ab54447. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

    Could you please let me know what exactly the problem was that you encountered (e.g. high background, no signal)? Based on that information I might be able to provide additional protocol suggestionsthat may help improve your results.

    I look forward to hear back from you.

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Frozen sections)
    Mouse Tissue sections (Lung w/ B16 cells)
    Lung w/ B16 cells
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Sep 17 2012


    Thank you for your reply. The code *** can be used for testing ab54447 in IHC-Fr. This code will expire on August 30th, 2012. I hope this helps, please let me know if you have any additional questions or concerns.

    Read More


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