• Product name

    Anti-Tyrosinase antibody [T311]
    See all Tyrosinase primary antibodies
  • Description

    Mouse monoclonal [T311] to Tyrosinase
  • Host species

  • Specificity

    Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein.

  • Positive control

    • Melanoma
  • General notes

    This product was changed from ascites to tissue culture supernatant on [20/07/17]. The following lots are from ascites and are still in stock as of [20/07/17] – [GR278593]. Lot numbers higher than [GR278593] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.



Our Abpromise guarantee covers the use of ab738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


  • Function

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • Involvement in disease

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • Sequence similarities

    Belongs to the tyrosinase family.
  • Cellular localization

    Melanosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATN antibody
    • CMM8 antibody
    • LB24 AB antibody
    • LB24-AB antibody
    • Monophenol monooxygenase antibody
    • OCA1 antibody
    • OCA1A antibody
    • OCAIA antibody
    • Oculocutaneous albinism IA antibody
    • SHEP3 antibody
    • SK29 AB antibody
    • SK29-AB antibody
    • Tumor rejection antigen AB antibody
    • TYR antibody
    • TYRO_HUMAN antibody
    • tyrosinase (oculocutaneous albinism IA) antibody
    • Tyrosinase antibody
    see all


  • Anti-Tyrosinase antibody [T311] (ab738) at 1/250 dilution + Human A375 whole cell lysate at 10000 cells

    HRP-conjugated Horse anti-mouse IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 65 kDa
    why is the actual band size different from the predicted?

    Exposure time: 10 minutes

    Blocked with 5% Milk for 1 hour at 20°C

    See Abreview

  • ab738 staining Tyrosinase in human neonatal foreskin (NHM) cells by Immunocytochemistry/ Immunofluorescence.
    NHM grown on coverslips were fixed and probed with anti-NCKX5-(I) (green) and ab738 (red). Merged images are shown.


This product has been referenced in:

  • Margaryan NV  et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . Read more (PubMed: 27011171) »
  • Allouche J  et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Human . Read more (PubMed: 26150484) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


Thank you for contacting us.

The IgG concentration of lot GR33380 is 66.7ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Western blot
Human Cell lysate - whole cell (A375 - melanoma cell line)
Loading amount
10000 cells
A375 - melanoma cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 31 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris - EDTA Buffer pH 9,0
Yes - Wash Buffer from Dako with Tween

Mr. Rudolf Jung

Verified customer

Submitted Sep 06 2011


Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab9788. Also, the antibody clearly stains IL1b positive cells in liver, spleen, pituitary and brain of endotoxin treated rats, so those tissues can be used as a positive control as well. Ab1872 - Placenta is recommended as a positive control. Also, caspase 1 is expressed in larger amounts in spleen and lung. Also found in liver, heart, skeletal muscle, kidney and testis. No expression in the brain. Ab738 - Melanoma is recommended as a positive control. Ab22601 - I couldn't find much information except for this: Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin. The gene expression is frequently activated in carcinoma derived cell lines.

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Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

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Thank you for your enquiry regarding ab738 and its use in WB. It has been reported in the literature that this clone T311 works in western however we have not tested it ourselves, I apologise for the inconvenience. We would recommend doing a preliminary experiment to determine the optimal dilution (a range of dilutions should be tried e.g 1:50-1:500) and we usually recommend to try BSA 5% in TBST (1hr, RT or 4C) for blocking. Please do not hesitate to contact us if you need any more advice or information or have any problems with your experiment,

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