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Tyrosinase Inhibitor Assay Kit (Colorimetric) (ab204715)

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  • Protocol Booklet
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Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715)

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: Inhibitor compounds

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    Overview

    • Product name

      Tyrosinase Inhibitor Assay Kit (Colorimetric)
      See all Tyrosinase kits
    • Detection method

      Colorimetric
    • Sample type

      Inhibitor compounds
    • Assay type

      Enzyme activity
    • Product overview

      Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at 510 nm. In the presence of kojic acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased. The assay is also adaptable to a 384-well format.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K575 Tyrosinase Inhibitor Screening Kit (Colorimetric). K575-100 is the same size as the 100 test size of ab204715.

      Tyrosinase or polyphenol oxidase (EC 1.14.18.1), is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling. Skin whitening and bleaching products utilize natural or synthetic tyrosinase inhibitors in order to lighten the skin color. Polyphenols, benzaldehyde derivatives, long-chain lipids, steroids, and natural compounds have been used as tyrosinase inhibitors.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      Inhibitor Control (Kojic Acid) 1 vial
      Tyrosinase (Lyophilized) 1 vial
      Tyrosinase Assay Buffer 1 x 25ml
      Tyrosinase Enhancer 1 x 500µl
      Tyrosinase Substrate 1 vial
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
    • Function

      This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
    • Involvement in disease

      Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
      Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
    • Sequence similarities

      Belongs to the tyrosinase family.
    • Cellular localization

      Melanosome membrane.
    • Target information above from: UniProt accession P14679 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • ATN
      • CMM8
      • LB24 AB
      • LB24-AB
      • Monophenol monooxygenase
      • OCA1
      • OCA1A
      • OCAIA
      • Oculocutaneous albinism IA
      • SHEP3
      • SK29 AB
      • SK29-AB
      • Tumor rejection antigen AB
      • TYR
      • TYRO_HUMAN
      • Tyrosinase
      • tyrosinase (oculocutaneous albinism IA)
      see all

    Images

    • Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715)
      Tyrosinase Inhibitor Screening Kit (Colorimetric) (ab204715)

      Inhibition of Tyrosinase Enzymatic Activity with Kojic Acid.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab204715? Please let us know so that we can cite the reference in this datasheet.

    ab204715 has been referenced in 1 publication.

    • Lee JY  et al. Tyrosinase-Targeting Gallacetophenone Inhibits Melanogenesis in Melanocytes and Human Skin-Equivalents. Int J Mol Sci 21:N/A (2020). PubMed: 32365630

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