Product nameAnti-Tyrosine Hydroxylase antibody
See all Tyrosine Hydroxylase primary antibodies
DescriptionRabbit polyclonal to Tyrosine Hydroxylase
Tested applicationsSuitable for: IHC-Fr, IHC-P, IHC-FrFl, ICC/IF, IHC-FoFr, WB, IP, ICCmore details
Species reactivityReacts with: Mouse, Rat, Goat, Cat, Human, Pig
Predicted to work with: Mammals
Full length SDS denatured protein (purified from pheochromocytoma) (Rat).
- IHC-P: Mouse and rat brain tissue. Rat dopaminergic neuronal tissue. ICC/IF: Human neurons. WB: Rat brain normal tissue lysate - total protein (ab29475), Mouse cell lysate (CATH.a neuron). PC-12 whole cell lysate. Rat caudate lysate. IHC-Fr: Mouse brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES
Concentration information loading...
PurityProtein A purified
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
- Anti-Tyrosine Hydroxylase antibody [EP1533Y] (Alexa Fluor® 488) (ab192463)
- Anti-Tyrosine Hydroxylase antibody [EP1533Y] (HRP) (ab193083)
- Anti-Tyrosine Hydroxylase antibody [EP1533Y] (Phycoerythrin) (ab209921)
- Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free (ab220218)
- Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
Our Abpromise guarantee covers the use of ab112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/750 - 1/1500.|
|IHC-FrFl||1/1000. PubMed: 222726|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20357108|
|WB||1/200. Predicted molecular weight: 60 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionPlays an important role in the physiology of adrenergic neurons.
Tissue specificityMainly expressed in the brain and adrenal glands.
PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Information by UniProt
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
Effects of hypercholesterolemia on tyrosine hydroxylase (TH)-immunoreactivity in striatum (NCP) and TH-positive nigral (SN) neurons in MPTP-treated mice.
Representative NCP (A-D) and SN (E-H) photographs from CS, HCD, MPTP and HCD+MPTP (left to right) groups showing TH-immunoreactivity.
Mice were anesthetized with chloral hydrate (350 mg/kg; i.p.) and perfused intracardially with phosphate buffered saline (PBS, 0.1 M; pH 7.4) followed by 4% w/v PFA in PBS. Brains were removed and kept overnight in the same fixative, transferred to 30% w/v sucrose solution. 20 μm thick coronal sections passing through the NCP and SN were taken using Cryotome in poly-L-lysine coated slides. The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH ab112 (1:700) in TBS, containing 2% donkey serum at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature. Colour development was performed by incubating the sections in DAB-liquid substrate system and then sections were washed, dehydrated, cleared in xylene, mounted in DPX and photographed under bright field illumination using a digital SLR camera.
Lateral localization of CB1-R-dense striosome-dendron bouquets and ventral tier SNpc enveloping CB1-R-immunoreactive axons through the substantia nigra (mouse).
An example of a TH- and CB1-R-positive dendron in the rostral SN is shown in Panel C.
Coronal vibratome sections (40 μm) or glycerol cryoprotected frozen sliding microtome sections (30 μm) were taken at levels extending from the prefrontal cortex to the ventral mesencephalon, and every 3rd to 6th section, depending on the experiment, was stained and imaged. Sections were washed in PBS-T (0.2% Triton X-100), blocked for 4 hours with 5% BSA in PBST, and incubated in primary antibodies overnight to 48 hours in a cold room on an orbital shaker.
ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
The image of Caudate putamen shows characteristic nerve fibre positivity
ab112 staining tyrosine hydroxulase in mouse brain tissue.
Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.
PFA-fixed, Triton X-100 permeabilized human neurons in cerebral organoid stained for Tyrosine Hydroxylase (green) using ab112 at 1/300 dilution in ICC/IF.
All lanes : Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution
All lanes : Mouse cell lysate (CATH.a neuron)
Lysates/proteins at 40 µg per lane.
All lanes : Goat Anti-Rabbit HRP
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 50 seconds
ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.
The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Predicted band size: 60 kDa
Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).
Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.
Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution + rat caudate lysate at 10 µg
Predicted band size: 60 kDa
ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections).
Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.
This product has been referenced in:
- Dolan CP et al. Axonal regrowth is impaired during digit tip regeneration in mice. Dev Biol 445:237-244 (2019). Read more (PubMed: 30458171) »
- Sang Q et al. Curcumin Protects an SH-SY5Y Cell Model of Parkinson's Disease Against Toxic Injury by Regulating HSP90. Cell Physiol Biochem 51:681-691 (2018). Read more (PubMed: 30463061) »