Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Sheep polyclonal to Tyrosine Hydroxylase
  • Host species

    Sheep
  • Tested applications

    Suitable for: IHC-FrFl, ICC/IF, IHC-Fr, WB, IP, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase. SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma (rat)
    Database link: P04177

  • Positive control

    • Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB.
  • General notes

    ab113 can be used as a marker for dopaminergic and noradrenergic neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/1000 - 1/2000.
ICC/IF 1/1000.
IHC-Fr 1/1000.
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • ab113 staining Tyrosine Hydroxylase in mouse brain tissue by Immunohistochemistry (Free Floating). Tissue was incubated with ab113 at a 1/1000 dilution for 48 hours at 4°C. The secondary used was a Dylight 594 conjugated donkey anti-sheep IgG (H+L) (ab96941) used at a 1/500 dilution.

    See Abreview

  • Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

  • ab113 at 1/2000 dilution staining rat brain cells (ab29475) by immunocyctochemistry. The antibody was incubated with the tissue for 48 hours and then bound antibody was detected using a biotinylated donkey anti-sheep antibody. The image shows the staining of free-floating 50 micron brainstem sections, labelling A5cell groups. The antibody also labels fine varicosities of axons.

     

    See Abreview

  • Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.

References

This product has been referenced in:

See all 34 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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1-2 of 2 Abreviews

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain slice)
Specification
brain slice

Dr. Xiaobin Liu

Verified customer

Submitted Jul 18 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (brain)
Specification
brain
Fixative
Paraformaldehyde

Prof. Jim Deuchars

Verified customer

Submitted May 16 2006

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