• Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Sheep polyclonal to Tyrosine Hydroxylase
  • Host species

  • Tested applications

    Suitable for: IHC-FrFl, ICC/IF, IHC-Fr, WB, IP, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase. SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma (rat)
    Database link: P04177

  • Positive control

    • Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB.
  • General notes

    ab113 can be used as a marker for dopaminergic and noradrenergic neurons.



Our Abpromise guarantee covers the use of ab113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/1000 - 1/2000.
ICC/IF 1/1000.
IHC-Fr 1/1000.
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.


  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all


  • ab113 staining Tyrosine Hydroxylase in mouse brain tissue by Immunohistochemistry (Free Floating). Tissue was incubated with ab113 at a 1/1000 dilution for 48 hours at 4°C. The secondary used was a Dylight 594 conjugated donkey anti-sheep IgG (H+L) (ab96941) used at a 1/500 dilution.

    See Abreview

  • Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

  • ab113 at 1/2000 dilution staining rat brain cells (ab29475) by immunocyctochemistry. The antibody was incubated with the tissue for 48 hours and then bound antibody was detected using a biotinylated donkey anti-sheep antibody. The image shows the staining of free-floating 50 micron brainstem sections, labelling A5cell groups. The antibody also labels fine varicosities of axons.


    See Abreview

  • Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.


This product has been referenced in:

See all 34 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A


Thank you for your email.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab113.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Thank you for your patience. I have received confirmation from the originator of the product and it seems that there is no specific data on whether the antibody recognizes all isoforms of histone H1, as we do not currently have access to the antigen. This data was originally determined via ELISA. I am sorry I can't be of more assistance in this occasion but should you require any further information, please do not hesitate to contact me.

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Dear Sir: Both of these antibodies can be used for immunocytochemistry and immunohistochemistry. The difference is that the NB300-108 is sensitive enough to stain fibers in the cerebral cortex. The cost of these two antibodies is $175. We would ship these antibodies, using dry ice ($10) and the delivery charge would be $60. This would be a total of $245 (USD). Delivery would take 2 business days to arrive to you from our establishment. I hope this reply answers all of your questions. Best Regards, Kate Lynott, DSO Novus Biologicals, Inc.

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The antibody should work for this application. For a reference, see: Lewis, D.A., et al. Four Isoforms of Tyrosine Hydroxylase are Expressed in Human Brain. 54(2): 477-492, 1993.

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raised against native rat - purified from pheochromocytoma, host species is sheep, can be used to detect all mammalian forms,WB gives a band at ~60 kDa [1:200 dilution (S35-protein A)], can be used in IP (1:10), IH, and IC (1:2000),supplied in 15 ug units at 100 ul, each. For more information, please check the www.novus-biolocals.com webpage (catalog # NB300-110).

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