Key features and details
- Chicken polyclonal to Tyrosine Hydroxylase
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat
- Isotype: IgY
Product nameAnti-Tyrosine Hydroxylase antibody
See all Tyrosine Hydroxylase primary antibodies
DescriptionChicken polyclonal to Tyrosine Hydroxylase
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat
Synthetic peptide within Human Tyrosine Hydroxylase aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- This antibody gave a positive result in IHC in the following FFPE tissue: Rat 6 week old brain. It also gave a positive result in ICC/IF in PC12 cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 3% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab134461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays an important role in the physiology of adrenergic neurons.
Tissue specificityMainly expressed in the brain and adrenal glands.
PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Information by UniProt
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
IHC image of Tyrosine Hydroxylase staining in Rat 6 week brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134461, 0.2µg/ml, for 15 mins at room temperature. A Goat anti-Chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab134461 has not yet been referenced specifically in any publications.