Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to Tyrosine Hydroxylase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 150-490 of Human Tyrosine Hydroxylase.

  • Positive control

    • NT2D1, PC-3, U87-MG, SK-N-SH, Mouse brain and PC-12 whole cell lysates; SK-N-SH cells; Human H661 xenograft and Rat hindlimb pad skin tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab137721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded H661 xenograft labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
  • Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS-PAGE
  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC-3 whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5 % SDS-PAGE
  • Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 58 kDa



    7.5 % SDS-PAGE
  • Immunofluorescent analysis of methanol-fixed SK-N-SH cells labelling Tyrosine Hydroxylase with ab137721 at 1/500 dilution (left panel) and co-stained with Hoechst 33343 (right panel). SK-N-SH cells were fixed in -20°C 100% MeOH for 5 min.
  • Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
  • Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.

References

This product has been referenced in:

  • Pyon WS  et al. An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue. Front Neuroanat 13:73 (2019). Read more (PubMed: 31379520) »
See 1 Publication for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Ciona intestinalis Embryo (larva)
Specification
larva

Abcam user community

Verified customer

Submitted Oct 18 2016

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