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  1. Link

    tyrosine-hydroxylase-antibody-ab137721.pdf

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Neuroscience Neurotransmitter Biogenic Amines Dopamine
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Anti-Tyrosine Hydroxylase antibody (ab137721)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137721)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)

Key features and details

  • Rabbit polyclonal to Tyrosine Hydroxylase
  • Suitable for: IHC-FoFr, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to Tyrosine Hydroxylase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 150-490 of Human Tyrosine Hydroxylase.

  • Positive control

    • NT2D1, PC-3, U87-MG, SK-N-SH, Mouse brain and PC-12 whole cell lysates; SK-N-SH cells; Human H661 xenograft and Rat hindlimb pad skin tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • SK-N-SH whole cell lysate (ab3956)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol.
Notes
IHC-FoFr
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7054 Human
    • Entrez Gene: 21823 Mouse
    • Entrez Gene: 25085 Rat
    • Omim: 191290 Human
    • SwissProt: P07101 Human
    • SwissProt: P24529 Mouse
    • SwissProt: P04177 Rat
    • Unigene: 435609 Human
    • Unigene: 1292 Mouse
    • Unigene: 11082 Rat
    see all
  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunohistochemical analysis of paraffin-embedded H661 xenograft labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS-PAGE
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
    All lanes : Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : PC-3 whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : SK-N-SH whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5 % SDS-PAGE
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 58 kDa



    7.5 % SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunofluorescent analysis of methanol-fixed SK-N-SH cells labelling Tyrosine Hydroxylase with ab137721 at 1/500 dilution (left panel) and co-stained with Hoechst 33343 (right panel). SK-N-SH cells were fixed in -20°C 100% MeOH for 5 min.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
    Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab137721? Please let us know so that we can cite the reference in this datasheet.

ab137721 has been referenced in 2 publications.

  • Pyon WS  et al. An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue. Front Neuroanat 13:73 (2019). PubMed: 31379520
  • Chiu HY  et al. Long-lasting alterations in 5-HT2A receptor after a binge regimen of methamphetamine in mice. Int J Neuropsychopharmacol 17:1647-58 (2014). PubMed: 24763081

Customer reviews and Q&As

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IHC - Wholemount abreview for Anti-Tyrosine Hydroxylase antibody

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Abreviews
Abreviews
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Application
IHC - Wholemount
Sample
Ciona intestinalis Embryo (larva)
Specification
larva
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Oct 18 2016

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