Anti-Tyrosine Hydroxylase antibody (ab6211)

Thank you for contacting Abcam.

I apologize for the delay, our laboratory was looking into the availability of the blocking peptide for ab6211.

We do not have the blocking peptide for ab6211 available separately. However, the peptide sequence is published: PRFIGRRQSLIEDARK, should you wish to pursue your own means of synthesizing the peptide. A 80-85% purity is generally good enough for blocking studies. Below is a link with a list of companies that provide peptide synthesis services:
http://www.peptideresource.com/custom-peptide.html

ab6211 recognizes amino acids 32-47 of Rat Tyrosine Hydroxylase, and is also reactive with mouse, guinea pig, and human tyrosine hydroxylase with predicted reactivity in chicken, chimpanzee, and macaque monkey. Thus, it should label total tyrosine hydroxylase through recognition of amino acids 32-47.

To our knowledge, ab6211 has not been tested in western blot. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. The reference number, or credit ID, is ******. Please refer to this number in any correspondence with the accounting department. I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.  

Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through your protocol, which looks fine to me. I appreciate the time that has been spent on these experiments and would be pleased to arrange a credit note or a replacement. Unfortunately the only lot of ab6211 we currently have in stock is the same your customer received. Therefore I can offer a replacement with one of the following alternative products, tested in Mouse and IHC-FoFR : ab41528, www.abcam.com/ab41528 ab51191, www.abcam.com/ab51191 ab112, www.abcam.com/ab112 ab113, www.abcam.com/ab113 I look forward to hearing from you with details of how you would like to proceed. Could you also please inform us that you added glycerol to ab6211 before storing at -20°C as recommended on the datasheet?

Thank you for your enquiry. Since you are seeing some specific staining in addition to this ubiquitous background staining, I am thinking this may be non-specific staining due to using too high a concentration of primary antibody. The recommendation on the datasheet is a starting point only, and I would recommend diluting this antibody out further, perhaps trying serial dilutions 1:2000 and 1:4000. In addition, I didn't see from your enquiry how you were incubating with the antibody. I would recommend an overnight incubation at 4C, as this will give the most specific signal. If you are still having problems with this antibody after following these suggestions, please let us know. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested this ourselves. We invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. I hope this information helps and please let me know how the ELISAs work.

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soona s any new information is brought to our attention. As far as we are aware, cross reactivity with human has not yet been tested for use with ab6211. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.