For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    tyrosine-hydroxylase-antibody-ab6211.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmitter Biogenic Amines Dopamine
Share by email

Anti-Tyrosine Hydroxylase antibody (ab6211)

  • Datasheet
Reviews (4)Q&A (8)References (52)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)

Key features and details

  • Rabbit polyclonal to Tyrosine Hydroxylase
  • Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Fr
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to Tyrosine Hydroxylase
  • Host species

    Rabbit
  • Specificity

    Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 1-100 conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
    Database link: P04177

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     

    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Primary antibody notes

    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)
  • Related Products

    • Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep (ab120212)
    • Normal Goat Serum (ab7481)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
Use at an assay dependent concentration.
WB
1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
ICC/IF
1/1000.
IHC-Fr (2)
Use at an assay dependent concentration.
Notes
IHC-FoFr
Use at an assay dependent concentration.
WB
1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
ICC/IF
1/1000.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 21823 Mouse
    • Entrez Gene: 25085 Rat
    • SwissProt: P24529 Mouse
    • SwissProt: P04177 Rat
    • Unigene: 1292 Mouse
    • Unigene: 11082 Rat
    • Alternative names

      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    Images

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom

      Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.

    • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)
      Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)

      ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    • Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
      Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
      Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg

      Performed under reducing conditions.

      Predicted band size: 42,53,84 kDa



      Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

    • Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
      Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)Image courtesy of QBM Cell Sciences, Ottawa, Canada
      ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)

      Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

    Protocols

    • Recommended IHC-Fr protocol with ab6211

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (52)

    Publishing research using ab6211? Please let us know so that we can cite the reference in this datasheet.

    ab6211 has been referenced in 52 publications.

    • Zhang H  et al. Levodopa affects spike and local field synchronisation in the pedunculopontine nucleus of a rat model of Parkinson's disease. Aging (Albany NY) 13:7314-7329 (2021). PubMed: 33639616
    • Zeng X  et al. Benefits of Iron Chelators in the Treatment of Parkinson's Disease. Neurochem Res 46:1239-1251 (2021). PubMed: 33646533
    • Li M  et al. Roles of Motor Cortex Neuron Classes in Reach-Related Modulation for Hemiparkinsonian Rats. Front Neurosci 15:645849 (2021). PubMed: 33986639
    • Han X  et al. Quercetin hinders microglial activation to alleviate neurotoxicity via the interplay between NLRP3 inflammasome and mitophagy. Redox Biol 44:102010 (2021). PubMed: 34082381
    • Nam IS  et al. Specific mutations in the genes of MC1R and TYR have an important influence on the determination of pheomelanin pigmentation in Korean native chickens. J Adv Vet Anim Res 8:266-273 (2021). PubMed: 34395597
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Zebra finch Tissue sections (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 17 2019

    Immunohistochemistry (Resin sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Resin sections)
    Sample
    Mouse Tissue sections (Retina)
    Specification
    Retina
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 27 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain-SNpc)
    Specification
    Brain-SNpc
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    Submitted Apr 13 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat mesencephalic brain)
    Specification
    Rat mesencephalic brain
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sophie Pezet

    Verified customer

    Submitted Apr 12 2006

    Question

    Product code: 6211

    Inquiry: I am currently trying to find a primary antibody for total tyrosine hydroxylase that has an associated blocking peptide available. The primary I have been trialing has no blocking peptide and the band is at an improper location. Does 6211 label total tyrosine hydroxylase and is there a blocking peptide available?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 04 2012

    Answer

    Thank you for contacting Abcam.

    I apologize for the delay, our laboratory was looking into the availability of the blocking peptide for ab6211.

    We do not have the blocking peptide for ab6211 available separately. However, the peptide sequence is published: PRFIGRRQSLIEDARK, should you wish to pursue your own means of synthesizing the peptide. A 80-85% purity is generally good enough for blocking studies. Below is a link with a list of companies that provide peptide synthesis services:
    http://www.peptideresource.com/custom-peptide.html

    ab6211 recognizes amino acids 32-47 of Rat Tyrosine Hydroxylase, and is also reactive with mouse, guinea pig, and human tyrosine hydroxylase with predicted reactivity in chicken, chimpanzee, and macaque monkey. Thus, it should label total tyrosine hydroxylase through recognition of amino acids 32-47.

    To our knowledge, ab6211 has not been tested in western blot. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 04 2012

    Question

    Thanks for your kindly reply, after I contacted with this customer, she would like to have a credit note. Therefore, would you please help this customer to create a credit note? Thanks for your kindly assistance.    

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. The reference number, or credit ID, is ******. Please refer to this number in any correspondence with the accounting department. I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.  

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Question

    LOT NUMBER 1077964 DESCRIPTION OF THE PROBLEM No staining SAMPLE Species: mouse What’s cell line or tissue: brain tissue PRIMARY ANTIBODY Species: rabbiy Reacts against: Tyrosine hydroxylase At what dilution(s) have you tested this antibody: 1:200/1:500/1:1000 Diluent buffer: 0.3% Triton X-100 Incubation time: overnight Incubation temperature: 4 degree What washing steps were done (which buffer, number of washes): PBS wash 3 time, each time 5 min ANTIBODY STORAGE CONDITIONS -20 FIXATION OF SAMPLE Yes or No : yes If yes, Fixative agent and concentration: paraformaldehyde Fixation time: perfusion and post-fix overnight Fixation temperature : 4 degree ANTIGEN RETRIEVAL no PERMEABILIZATION STEP Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? yes Permeabilizing agent and concentration: PBS-0.1% Triton for 15 min at RT BLOCKING CONDITIONS Concentration: 10% donkey serum/PBS-0.05% Tween Blocking time : 1hr Blocking temperature: RT SECONDARY ANTIBODY Species: Alexa 594 donkey anti-rabbit Reacts against which species: At what dilution(s) have you tested this antibody: 1:200/1:500 Diluent buffer: 0.3% Triton X-100 Incubation time: overnight What washing steps were done (which buffer, number of washes): PBS wash 3 time, each time 5 min ˙Fluorochrome or enzyme conjugate (eg: FITC, HRP, AP, biotin…etc): Alexa 594 donkey anti-rabbit Do you know whether the problems you are experiencing come from the secondary? NO HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 7 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? NO

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2011

    Answer

    Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through your protocol, which looks fine to me. I appreciate the time that has been spent on these experiments and would be pleased to arrange a credit note or a replacement. Unfortunately the only lot of ab6211 we currently have in stock is the same your customer received. Therefore I can offer a replacement with one of the following alternative products, tested in Mouse and IHC-FoFR : ab41528, www.abcam.com/ab41528 ab51191, www.abcam.com/ab51191 ab112, www.abcam.com/ab112 ab113, www.abcam.com/ab113 I look forward to hearing from you with details of how you would like to proceed. Could you also please inform us that you added glycerol to ab6211 before storing at -20°C as recommended on the datasheet?

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2011

    Question

    I recently purchased one of your products ab6211, a Tyrosine Hydroxylase antibody, and I'm having trouble with doing IHC on dopaminergic neurons. When I did a double staining of these dopaminergic with both ab6211 and the monoclonal anti-tyrosine hydroxlase TH-2 antibody from sigma, I got different results. The sigma antibody showed very specific staining, outlining specific areas of cells, whereas ab6211 showed a lot of aspecific spotty background staining. I used a dilution of 1:1000 (as recommended) for ab6211 and 1:8000 for the sigma antibody. The secondary antibody used for the ab6211 was Cy5 conjugated AffiniPUre Donkey anti-Rabbit IgG from Jackson Labs. I've attached a picture of a confocal image taken of these cells. Red is staining with TH-2, Green is ab6211, blue is DAPI. I would appreciate any advice on what to do to improve IHC staining with ab6211.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2006

    Answer

    Thank you for your enquiry. Since you are seeing some specific staining in addition to this ubiquitous background staining, I am thinking this may be non-specific staining due to using too high a concentration of primary antibody. The recommendation on the datasheet is a starting point only, and I would recommend diluting this antibody out further, perhaps trying serial dilutions 1:2000 and 1:4000. In addition, I didn't see from your enquiry how you were incubating with the antibody. I would recommend an overnight incubation at 4C, as this will give the most specific signal. If you are still having problems with this antibody after following these suggestions, please let us know. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Nov 13 2006

    Question

    In our lab we use a phenol-ethanol supernatant obtained after precipitation of DNA with ethanol, we wonder if we could use this supernatant for the ELISA with acetilcholine and noradrenaline antibodies. The protocols displayed in the internet page, have been usefull. I look forward to your reply.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 01 2006

    Answer

    Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested this ourselves. We invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. I hope this information helps and please let me know how the ELISAs work.

    Read More

    Abcam Scientific Support

    Answered on Sep 05 2006

    Question

    I am interested in purchasing the antibody -- Rabbit polyclonal to Tyrosine Hydroxylase (Cat. # 6211). Here I inquire about whether the ab6211 can react with human tissue or not? If not yet tested in your company, could you kindly send me some trial sample, so, I can make it for testing.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2004

    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soona s any new information is brought to our attention. As far as we are aware, cross reactivity with human has not yet been tested for use with ab6211. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2004

    1-10 of 12 Abreviews or Q&A

    •  Previous
    • 1
    • 2
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.