• Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to Tyrosine Hydroxylase
  • Host species

  • Specificity

    Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
  • Tested applications

    Suitable for: IHC-FoFr, ELISA, WB, ICC/IF, IHC (PFA fixed), IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Guinea pig, Human
    Predicted to work with: Chicken, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 32-47 conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).


    Database link: P04177

  • Positive control

    • Mouse or rat brain containing dopaminergic neurons. Ventral mesencephalic neurons (mouse embryonic day 14).
  • General notes


    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.



Our Abpromise guarantee covers the use of ab6211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/500. PubMed: 19630993
ELISA Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
ICC/IF 1/1000.
IHC (PFA fixed) 1/1000.
IHC-Fr Use at an assay dependent concentration.


  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all


  • Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.

  • ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg

    Performed under reducing conditions.

    Predicted band size: 42,53,84 kDa

    Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

  • ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
  • Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.


This product has been referenced in:

See all 33 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Immunohistochemistry free floating
Zebra finch Tissue sections (Brain)

Abcam user community

Verified customer

Submitted Jan 17 2019

Immunohistochemistry (Resin sections)
Mouse Tissue sections (Retina)

Abcam user community

Verified customer

Submitted Jun 27 2016

Immunohistochemistry (Frozen sections)
Human Tissue sections (Brain-SNpc)
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 13 2012

Immunohistochemistry (Frozen sections)
Rat Tissue sections (Rat mesencephalic brain)
Rat mesencephalic brain

Dr. Sophie Pezet

Verified customer

Submitted Apr 12 2006

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