Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Chicken polyclonal to Tyrosine Hydroxylase
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-FoFr, IHC-FrFl, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.

Properties

Applications

Our Abpromise guarantee covers the use of ab76442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000. using 2% paraformaldehyde-fixed tissues or cells.
IHC-P 1/1000 - 1/2000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20653037
IHC-FrFl 1/1000.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Plays an important role in the physiology of adrenergic neurons.
    • Tissue specificity

      Mainly expressed in the brain and adrenal glands.
    • Pathway

      Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
    • Involvement in disease

      Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
      Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
    • Sequence similarities

      Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    Images

    • AAV9 and AAVDJ8 transduce DA neurons and astrocytes of the substantia nigra

      10X objective representative images of SN from 6-week tissue immunostained for TH (cyan) to visualize viral GFP expression (native) in the SN pars compacta (white outline) and SN pars reticulata for AAV2/1 (A), AAVDJ8 (B), and AAV9 (C) injected brains. (E) AAV9 and (F) AAVDJ8 100X objective representative images immunostained for TH and S100β (red) within the SNpc. at 6-weeks post ICV. (H) Representative, three dimensional (3D) 40X objective montage image of clarified SN tissue from a AAVDJ8–GFP infected brain, immunostained for TH (cyan), GFAP (red) and GFP (green) in XYZ and XZ volumetric planes.

      TH = Tyrosine Hydroxylase. 

      Tyrosine Hydroxylase is detected using ab76442 at 1/200 dilution in frozen sections of mouse substantia nigra.

      (From Figure 6 of Hammond et al)

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).

    • Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).

    • ab76442 staining Tyrosine Hydroxylase in murine brain tissue by Immunohistochemistry (Frozen sections).

      Tissue was fixed with paraformaldehyde, permeabilizd by Triton X-100 and blocked with 3% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour at 37°C. ab6753 HRP-conjugated rabbit anti-chicken polyclonal IgG (ab6753) (1/500) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:

    • Brown AG  et al. Impaired chemoreflex correlates with decreased c-Fos in respiratory brainstem centers of the streptozotocin-induced Alzheimer's disease rat model. Exp Neurol 311:285-292 (2019). Read more (PubMed: 30359566) »
    • Meng F  et al. CD73-derived adenosine controls inflammation and neurodegeneration by modulating dopamine signalling. Brain 142:700-718 (2019). Read more (PubMed: 30689733) »
    See all 53 Publications for this product

    Customer reviews and Q&As

    1-10 of 10 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (dorsal root ganglion)
    Specification
    dorsal root ganglion

    Dr. J. Sebastián Jara

    Verified customer

    Submitted Aug 13 2019

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS + 0.2% triton + 4% BSA
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Formaldehyde

    Dr. Alexandre Magno

    Verified customer

    Submitted Jul 20 2018

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Mr. J. Y. Seo

    Verified customer

    Submitted Mar 30 2018

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - Triton X100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 15 2015

    Answer

    Vielen Dank für Ihre Anfrage.
    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 90.000 Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette Teströhrchen zu lagern. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben und er innerhalb der 180 Tage Garantiezeit reklamiert wird. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wirden soll, können wir Ihnen aber unseren 100% Abreview Discount anbieten. Bitte lassen Sie mich wissen, ob dies auf Ihr Experiment zutrifft.
    Falls der Antikörper in einer getesteten Anwendung oder Spezies verwendet wird, möchten wir Sie außerdem einladen unser Abreview-System zu verwenden.
    Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.
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    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 0,1%
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Sep 06 2012

    Answer

    Thank you for your inquiry.

    I can confirm that the authors of this publication (Mousa et al., 2011) acquired the testinginformation from theproducing labof this antibody directly.

    I suggest thereforeto cite this publication for the information that is asked by The Journal of Comparative Neurology.

    "Chicken polyclonal anti-TH (Abcam, Cambridge, UK)

    detects an expected single band at 59 kDa in Western blot

    in rat (manufacturer’s technical information) and mouse

    (Samaco et al., 2009; Mousa et al., 2010) brain. In addition,

    the company showed that the immunolabeling pattern

    of TH was consistent with specific staining of catecholaminergic

    neuronal cell bodies in the rat superior cervical ganglion,

    and the staining of sympathetic and noradrenergic

    neurons in the rat central nervous system (CNS) as well as

    dopaminergic terminals in the rat caudate-putamen nucleus.

    Immunolabeling with the TH antibody was completely

    abolished by preabsorption with 10 lg/ml of the

    cognate peptide (manufacturer’s technical information)."

    I hope this information is re-assuring you that ab76442 was tested as described and wish you success with your publication.

    Read More

    Answer


    Les 2 anticorps anti-Tyrosine Hydroxylaseab76442 et ab53417 (retiré du catalogue) sont tous les 2 des IgY dirigés contre les mêmes peptides immunogènes.Cependant nous ne pouvons dire que ce sont exactement les mêmes carils ne proviennent pas du même laboratoire de production.

    Read More
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain, 20 µm frozen section)
    Specification
    brain, 20 µm frozen section

    Abcam user community

    Verified customer

    Submitted Jun 09 2011

    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain

    Dorothee Domenger

    Verified customer

    Submitted Jul 15 2010

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