Anti-Tyrosine Hydroxylase antibody (ab76442)
Key features and details
- Chicken polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-P, IHC-FrFl
- Reacts with: Mouse
- Isotype: IgY
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Overview
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Product name
Anti-Tyrosine Hydroxylase antibody
See all Tyrosine Hydroxylase primary antibodies -
Description
Chicken polyclonal to Tyrosine Hydroxylase -
Host species
Chicken -
Tested applications
Suitable for: IHC-P, IHC-FrFlmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.02% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Two different affinity-purified anti-peptide antibodies were combined to make this product. After repeated injections of synthetic peptides into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized. The concentrations of both antibodies were 100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200 ug/ml. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/1000 - 1/2000.
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| IHC-FrFl | (3) |
1/1000.
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| Notes |
|---|
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IHC-P
1/1000 - 1/2000. |
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IHC-FrFl
1/1000. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 21823 Mouse
- SwissProt: P24529 Mouse
- Unigene: 1292 Mouse
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Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
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Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
Datasheets and documents
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SDS download
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Datasheet download
References (93)
ab76442 has been referenced in 93 publications.
- Campbell-Thompson M & Tang SC Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes. Front Endocrinol (Lausanne) 12:644826 (2021). PubMed: 33981285
- Courtland JL et al. Genetic disruption of WASHC4 drives endo-lysosomal dysfunction and cognitive-movement impairments in mice and humans. Elife 10:N/A (2021). PubMed: 33749590
- Scherschel K et al. Characterization of the HCN Interaction Partner TRIP8b/PEX5R in the Intracardiac Nervous System of TRIP8b-Deficient and Wild-Type Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33946275
- Jasmin M et al. Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson's disease features. EMBO J 40:e105537 (2021). PubMed: 33351190
- Liu X et al. Reduced erythrocytic CHCHD2 mRNA is associated with brain pathology of Parkinson's disease. Acta Neuropathol Commun 9:37 (2021). PubMed: 33685516