Anti-Tyrosine Hydroxylase antibody (ab76442)
- Datasheet
- References (53)
- Protocols
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody
See all Tyrosine Hydroxylase primary antibodies -
Description
Chicken polyclonal to Tyrosine Hydroxylase -
Host species
Chicken -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-FoFr, IHC-FrFl, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Two different affinity-purified anti-peptide antibodies were combined to make this product. After repeated injections of synthetic peptides into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized. The concentrations of both antibodies were 100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200 ug/ml. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000 - 1/2000. using 2% paraformaldehyde-fixed tissues or cells. | |
| IHC-P | 1/1000 - 1/2000. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20653037 | |
| IHC-FrFl | 1/1000. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)Hammond, S.L. et al PLoS One. 2017 Dec 15;12(12):e0188830. doi: 10.1371/journal.pone.0188830. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/AAV9 and AAVDJ8 transduce DA neurons and astrocytes of the substantia nigra
10X objective representative images of SN from 6-week tissue immunostained for TH (cyan) to visualize viral GFP expression (native) in the SN pars compacta (white outline) and SN pars reticulata for AAV2/1 (A), AAVDJ8 (B), and AAV9 (C) injected brains. (E) AAV9 and (F) AAVDJ8 100X objective representative images immunostained for TH and S100β (red) within the SNpc. at 6-weeks post ICV. (H) Representative, three dimensional (3D) 40X objective montage image of clarified SN tissue from a AAVDJ8–GFP infected brain, immunostained for TH (cyan), GFAP (red) and GFP (green) in XYZ and XZ volumetric planes.
TH = Tyrosine Hydroxylase.
Tyrosine Hydroxylase is detected using ab76442 at 1/200 dilution in frozen sections of mouse substantia nigra.
(From Figure 6 of Hammond et al)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
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Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
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Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)This image is courtesy of an anonymous Abreviewab76442 staining Tyrosine Hydroxylase in murine brain tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed with paraformaldehyde, permeabilizd by Triton X-100 and blocked with 3% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour at 37°C. ab6753 HRP-conjugated rabbit anti-chicken polyclonal IgG (ab6753) (1/500) was used as the secondary antibody.
References
This product has been referenced in:
- Brown AG et al. Impaired chemoreflex correlates with decreased c-Fos in respiratory brainstem centers of the streptozotocin-induced Alzheimer's disease rat model. Exp Neurol 311:285-292 (2019). Read more (PubMed: 30359566) »
- Meng F et al. CD73-derived adenosine controls inflammation and neurodegeneration by modulating dopamine signalling. Brain 142:700-718 (2019). Read more (PubMed: 30689733) »
