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  1. Link

    tyrosine-hydroxylase-antibody-ab76442.pdf

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Anti-Tyrosine Hydroxylase antibody (ab76442)

  • Datasheet
  • SDS
Reviews (11)Q&A (3)References (94)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
  • Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)

Key features and details

  • Chicken polyclonal to Tyrosine Hydroxylase
  • Suitable for: IHC-P, IHC-FrFl
  • Reacts with: Mouse
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Tyrosine Hydroxylase antibody [EP1533Y] - Neuronal Marker (ab75875)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Chicken polyclonal to Tyrosine Hydroxylase
  • Host species

    Chicken
  • Tested applications

    Suitable for: IHC-P, IHC-FrFlmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.02% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Two different affinity-purified anti-peptide antibodies were combined to make this product. After repeated injections of synthetic peptides into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized. The concentrations of both antibodies were 100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200 ug/ml.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
    • Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    • Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/1000 - 1/2000.
IHC-FrFl (3)
1/1000.
Notes
IHC-P
1/1000 - 1/2000.
IHC-FrFl
1/1000.
Application notes
Is unsuitable for WB.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 21823 Mouse
    • SwissProt: P24529 Mouse
    • Unigene: 1292 Mouse
    • Alternative names

      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).

    • Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)
      Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)

      Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (94)

    Publishing research using ab76442? Please let us know so that we can cite the reference in this datasheet.

    ab76442 has been referenced in 94 publications.

    • Campbell-Thompson M & Tang SC Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes. Front Endocrinol (Lausanne) 12:644826 (2021). PubMed: 33981285
    • Courtland JL  et al. Genetic disruption of WASHC4 drives endo-lysosomal dysfunction and cognitive-movement impairments in mice and humans. Elife 10:N/A (2021). PubMed: 33749590
    • Scherschel K  et al. Characterization of the HCN Interaction Partner TRIP8b/PEX5R in the Intracardiac Nervous System of TRIP8b-Deficient and Wild-Type Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33946275
    • Jasmin M  et al. Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson's disease features. EMBO J 40:e105537 (2021). PubMed: 33351190
    • Liu X  et al. Reduced erythrocytic CHCHD2 mRNA is associated with brain pathology of Parkinson's disease. Acta Neuropathol Commun 9:37 (2021). PubMed: 33685516
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA pH=9
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 19°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Joana Lama

    Verified customer

    Submitted Feb 23 2022

    Immunohistochemistry free floating abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Daniel Lustberg

    Verified customer

    Submitted Aug 21 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Zebrafish Cell (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Lindsay Zentveld

    Verified customer

    Submitted Jan 22 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - 0.05% tritonX
    Specification
    brain tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 19 2019

    IHC - Wholemount abreview for Anti-Tyrosine Hydroxylase antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (dorsal root ganglion)
    Specification
    dorsal root ganglion
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. J. Sebastián Jara

    Verified customer

    Submitted Aug 13 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS + 0.2% triton + 4% BSA
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Alexandre Magno

    Verified customer

    Submitted Jul 20 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. J. Y. Seo

    Verified customer

    Submitted Mar 30 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - Triton X100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 15 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 0,1%
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 06 2012

    Question

    Dear Sir or Madame, I was wondering if you might sent us a small sample to test the anti-tyrosine hydroxylase antibody (Chicken)?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2012

    Answer

    Vielen Dank für Ihre Anfrage.
    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 90.000 Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette Teströhrchen zu lagern. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben und er innerhalb der 180 Tage Garantiezeit reklamiert wird. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wirden soll, können wir Ihnen aber unseren 100% Abreview Discount anbieten. Bitte lassen Sie mich wissen, ob dies auf Ihr Experiment zutrifft.
    Falls der Antikörper in einer getesteten Anwendung oder Spezies verwendet wird, möchten wir Sie außerdem einladen unser Abreview-System zu verwenden.
    Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.
    Unter der folgenden URL können Sie mehr über unser Abreview-System zu erfahren:
    https://www.abcam.com/abreviews
    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Sep 20 2012

    1-10 of 14 Abreviews or Q&A

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