For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    tyrosine-hydroxylase-antibody-ep1532y-ab137869.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmitter Biogenic Amines Dopamine
Share by email
RecombinantRabMAb

Recombinant Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (27)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
  • Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1532Y] to Tyrosine Hydroxylase - Neuronal Marker
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

You may also be interested in

Stain
Product image
Fontana-Masson Stain Kit (Melanin Stain) (ab150669)
Primary
Product image
HRP Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab207673)
Primary
Product image
Anti-VGLUT2 antibody [EPR21085] (ab216463)

View more associated products

Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody [EP1532Y]
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit monoclonal [EP1532Y] to Tyrosine Hydroxylase - Neuronal Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • PC12 cell lysate; Rat glial tumor cell line; Rat cerebral cortex; Mouse cerebral cortex; SH-SY5Y.
  • General notes

    The human recommendation is based on the WB result. This antibody may not be suitable for IHC with human samples.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1532Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • HRP Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab207673)
    • Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free (ab220218)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

For unpurified use at 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/5000. Predicted molecular weight: 58 kDa.

For unpurified use at 1/10000 - 1/50000.

IHC-P (3)
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/50.

For unpurified use at 1/1000.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Predicted molecular weight: 58 kDa.

For unpurified use at 1/10000 - 1/50000.

IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocol.

ICC/IF
1/100 - 1/250.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7054 Human
    • Entrez Gene: 21823 Mouse
    • Entrez Gene: 25085 Rat
    • Omim: 191290 Human
    • SwissProt: P07101 Human
    • SwissProt: P24529 Mouse
    • SwissProt: P04177 Rat
    • Unigene: 435609 Human
    • Unigene: 1292 Mouse
    • Unigene: 11082 Rat
    see all
  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1:500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling Tyrosine Hydroxylase with Purified ab137869 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Tyrosine Hydroxylase with purified ab137869 at 1/50 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    All lanes : Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 0.03 µg/ml (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1:500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)Image courtesy of Carl Hobbs, Kings College London, U.K.

    ab137869 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/800. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)Image courtesy of Carl Hobbs, Kings College London, U.K.

    ab137869 staining Tyrosine Hydroxylase in rat brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/1000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

    See Abreview

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 0.1 µg/ml (purified) + Human adrenal gland lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa



    Blocking and diluting buffer : 5% NFDM/TBST

  • Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

    Overlay histogram showing SHSY-5Y cells stained with ab137869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137869, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

     

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 1/100000 dilution (unpurified) + PC12 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 58 kDa

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)This image is courtesy of an Abreview submitted by Andre Antunes
    All lanes : Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 1/5000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate - transduced with AAV vector expressing human TH
    Lane 2 : SH-SY5Y cell lysate - non-infected

    Lysates/proteins at 20000 cells per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa


    Exposure time: 1 second


    Blocked with 5% milk for 1 hour at 25°C.

    See Abreview

  • Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
    Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (27)

Publishing research using ab137869? Please let us know so that we can cite the reference in this datasheet.

ab137869 has been referenced in 27 publications.

  • Qian X  et al. Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal Cancer. Front Oncol 11:655861 (2021). PubMed: 33898321
  • Sun X  et al. Comparison of the expression and toxicity of AAV2/9 carrying the human A53T a-synuclein gene in presence or absence of WPRE. Heliyon 7:e06302 (2021). PubMed: 33665452
  • Crane-Smith Z  et al. A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development. Front Cell Dev Biol 9:595744 (2021). PubMed: 33869166
  • Sass F  et al. TFEB deficiency attenuates mitochondrial degradation upon brown adipose tissue whitening at thermoneutrality. Mol Metab 47:101173 (2021). PubMed: 33516944
  • Uceda DE  et al. Accumulation of Pericardial Fat Is Associated With Alterations in Heart Rate Variability Patterns in Hypercholesterolemic Pigs. Circ Arrhythm Electrophysiol 13:e007614 (2020). PubMed: 32189516
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E14.5 brain)
Permeabilization
Yes - Triton
Specification
E14.5 brain
Blocking step
Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

IHC - Wholemount abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 whole embryo)
Specification
E12.5 whole embryo
Read More

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

Western blot abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Tissue lysate - whole (Left Ventricle)
Gel Running Conditions
Reduced Denaturing (4% loading - 10% resolving SDS-PAGE)
Loading amount
5 µg
Specification
Left Ventricle
Blocking step
Commercial blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Read More

Dr. Juan Estrada

Verified customer

Submitted Apr 05 2016

Western blot abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (SH-SY5Y cell line)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
20000 cells
Treatment
Transduced with AAV vector expressing human TH
Specification
SH-SY5Y cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Andre Antunes

Verified customer

Submitted Nov 12 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Myocardium)
Specification
Myocardium
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 04 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 04 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
10mg/mL BSA & 5% Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Dog Tissue sections (Atria)
Specification
Atria
Permeabilization
Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
Fixative
Paraformaldehyde
Read More

Dr. Juan Estrada

Verified customer

Submitted Oct 24 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.