Recombinant Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1532Y] to Tyrosine Hydroxylase
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody [EP1532Y]
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit monoclonal [EP1532Y] to Tyrosine Hydroxylase -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide within Human Tyrosine Hydroxylase aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- PC12 cell lysate; Rat glial tumor cell line; Rat cerebral cortex; Mouse cerebral cortex; SH-SY5Y.
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General notes
The human recommendation is based on the WB result. This antibody may not be suitable for IHC with human samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1532Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/5000. Predicted molecular weight: 58 kDa. For unpurified use at 1/10000 - 1/50000. |
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Flow Cyt | 1/50. For unpurified use at 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/250. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1:500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling Tyrosine Hydroxylase with Purified ab137869 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Tyrosine Hydroxylase with purified ab137869 at 1:50 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 0.03 µg/ml (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1:500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)Image courtesy of Carl Hobbs, Kings College London, U.K.
ab137869 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/800. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)Image courtesy of Carl Hobbs, Kings College London, U.K.
ab137869 staining Tyrosine Hydroxylase in rat brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21ºC followed by incubation with the primary antibody for 2 hours at 1/1000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.
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Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 0.1 µg/ml (purified) + Human adrenal gland lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaBlocking and diluting buffer : 5% NFDM/TBST
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Overlay histogram showing SHSY-5Y cells stained with ab137869 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137869, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 1/100000 dilution (unpurified) + PC12 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa -
Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869)This image is courtesy of an Abreview submitted by Andre AntunesAll lanes : Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab137869) at 1/5000 dilution (unpurified)
Lane 1 : SH-SY5Y cell lysate - transduced with AAV vector expressing human TH
Lane 2 : SH-SY5Y cell lysate - non-infected
Lysates/proteins at 20000 cells per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 1 secondBlocked with 5% milk for 1 hour at 25°C.
Protocols
References (12)
ab137869 has been referenced in 12 publications.
- Madrazo I et al. Transplantation of Human Neural Progenitor Cells (NPC) into Putamina of Parkinsonian Patients: A Case Series Study, Safety and Efficacy Four Years after Surgery. Cell Transplant 28:269-285 (2019). PubMed: 30574805
- Arotcarena ML et al. Transcription factor EB overexpression prevents neurodegeneration in experimental synucleinopathies. JCI Insight 4:N/A (2019). PubMed: 31434803
- Kiss A & Osacka J Spatial relationship between the c-Fos distribution and enkephalinergic, substance P, and tyrosine hydroxylase innervation fields after acute treatment with neuroleptics olanzapine, amisulpride, quetiapine, and aripiprazole in the rat septum. Endocr Regul 53:165-177 (2019). PubMed: 31517634
- Emrick JJ et al. Tissue-specific contributions of Tmem79 to atopic dermatitis and mast cell-mediated histaminergic itch. Proc Natl Acad Sci U S A 115:E12091-E12100 (2018). PubMed: 30463955
- Zhang M et al. Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload. J Cell Mol Med N/A:N/A (2018). PubMed: 30411499