Western blot abreview for Anti-Tyrosine Hydroxylase antibody [EP1532Y]

Western blot

Dog Tissue lysate - whole (Left Ventricle)

Reduced Denaturing (4% loading - 10% resolving SDS-PAGE)

5 µg

Left Ventricle

Commercial blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Commercial blocking solution

1/5000

1/20000

Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Advansta WesternBright Quantum ECL

2 minute(s) and 0 second(s)

Specific: 56 kDa Non-specific: 52 kDa

Molecular weight ladders (lanes 1 & 10 BioRad Prestained Protein Standards MW range 250-10) were visualized with ECL following incubation with mouse monoclonal HRP-conjugate raised against blue dye (Rockland Immunochemicals Inc). This step was performed following ECL exposure and image acquisition, using a CCD-camera, to capture tyrosine hydroxylase bands (lanes 2-8). Molecular weight ladder images were aligned and overlayed with the tyrosine hydroxylase image. Molecular weights of bands were measured automatically with GeneTools in the Syngene computer software package. We believe that both bands are specific for tyrosine hydroxylase, as primary anti-sera from other companies also identified these two bands and immunohistochemistry experiments label only nerve fiber structures. Perhaps one of the two bands represents a post-translational modification. MW ladders below 37kDa were not resolved under the running conditions and resolving gel % used in this experiment.