Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 whole embryo)
Specification
E12.5 whole embryo
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x + 0.5% Gelatin + 0.5% triton
Dilution
1/500
Secondary antibody
Dilution
1/400
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
Incubated for 7 days at 37°C in primary antibody
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
DR. Alain Chedotal
Verified customer
Submitted Nov 23 2020