Recombinant Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
![Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)](https://www.abcam.com/ps/products/75/ab75875/Images/ab75875-411500-anti-tyrosine-hydroxylase-antibody-ep1533y-westernblot-human-mouse-rat.jpg "Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: Flow Cyt (Intra), WB, ICC
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody [EP1533Y]
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICCmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab183303) -
Positive control
- WB: Human adrenal gland lysate. SH-SY5Y whole cell lysate. Mouse and rat brain lysate. PC-12 whole cell lysate. IHC-P: Human and mouse cerebral cortex tissue, Rat and mouse brain tissue. ICC: Neuro-2a and mouse midbrain neuron cells. Flow Cyt (intra): SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1533Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000. Predicted molecular weight: 59 kDa.Can be blocked with Tyrosine Hydroxylase peptide (ab183303).
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| ICC |
1/50.
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| Notes |
|---|
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000. Predicted molecular weight: 59 kDa.Can be blocked with Tyrosine Hydroxylase peptide (ab183303). |
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ICC
1/50. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)All lanes : Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) at 1/1000 dilution (Purified)
Lane 1 : Human adrenal gland lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa -
![Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)](https://www.abcam.com/ps/products/75/ab75875/Images/ab75875-11-anti-tyrosine-hydroxylase-antibody-ep1533y-immunocytochemistry-mouse-midbrain-neuron.jpg)
Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse midbrain neuron cells labelling Tyrosine Hydroxylase with ab75875 at 1/10 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary midbrain neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
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![Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)](https://www.abcam.com/ps/products/75/ab75875/Images/ab75875-9-anti-tyrosine-hydroxylase-antibody-ep1533y-immunocytochemistry-neuro-2a-mouse.jpg)
Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/50 dilution (2.18 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)](https://www.abcam.com/ps/products/75/ab75875/Images/ab75875-10-anti-tyrosine-hydroxylase-antibody-ep1533y-flowcytometry-sh-sy5y-human.jpg)
Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/20 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)](https://www.abcam.com/ps/products/75/ab75875/Images/ab75875-13-benefits-of-recombinant-antibodies.png)
Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab75875 has been referenced in 32 publications.
- Ma J et al. EphA1 Activation Induces Neuropathological Changes in a Mouse Model of Parkinson's Disease Through the CXCL12/CXCR4 Signaling Pathway. Mol Neurobiol 58:913-925 (2021). PubMed: 33057926
- Bhattarai Y et al. Role of gut microbiota in regulating gastrointestinal dysfunction and motor symptoms in a mouse model of Parkinson's disease. Gut Microbes 13:1866974 (2021). PubMed: 33459114
- Henriques F et al. Single-Cell RNA Profiling Reveals Adipocyte to Macrophage Signaling Sufficient to Enhance Thermogenesis. Cell Rep 32:107998 (2020). PubMed: 32755590
- Faivre F et al. Ablation of the tail of the ventral tegmental area compensates symptoms in an experimental model of Parkinson's disease. Neurobiol Dis 139:104818 (2020). PubMed: 32087289
- Noonong K et al. Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP+-Induced Mouse and Cellular Models of Parkinson's Disease. Front Neurosci 14:575459 (2020). PubMed: 33408606