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  1. Link

    tyrosine-hydroxylase-antibody-ep1533y-ab75875.pdf

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Neuroscience Neurotransmitter Biogenic Amines Dopamine
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RecombinantRabMAb

Recombinant Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

  • Datasheet
  • SDS
Reviews (7)Q&A (2)References (32)

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Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
  • Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
  • Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase - Neuronal Marker
  • Suitable for: Flow Cyt (Intra), WB, ICC
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody [EP1533Y]
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase - Neuronal Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICCmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab183303)

  • Positive control

    • WB: Human adrenal gland lysate. SH-SY5Y whole cell lysate. Mouse and rat brain lysate. PC-12 whole cell lysate. IHC-P: Human and mouse cerebral cortex tissue, Rat and mouse brain tissue. ICC: Neuro-2a and mouse midbrain neuron cells. Flow Cyt (intra): SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1533Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • HRP Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab193083)
    • PE Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab209921)
    • Anti-Tyrosine Hydroxylase antibody [EP1533Y] - BSA and Azide free (ab219729)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • Tyrosine Hydroxylase peptide (ab183303)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Predicted molecular weight: 59 kDa.Can be blocked with Tyrosine Hydroxylase peptide (ab183303).
ICC
1/50.
Notes
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000. Predicted molecular weight: 59 kDa.Can be blocked with Tyrosine Hydroxylase peptide (ab183303).
ICC
1/50.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7054 Human
    • Entrez Gene: 21823 Mouse
    • Entrez Gene: 25085 Rat
    • Omim: 191290 Human
    • SwissProt: P07101 Human
    • SwissProt: P24529 Mouse
    • SwissProt: P04177 Rat
    • Unigene: 435609 Human
    • Unigene: 1292 Mouse
    • Unigene: 11082 Rat
    see all
  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    All lanes : Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875) at 1/1000 dilution (Purified)

    Lane 1 : Human adrenal gland lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse midbrain neuron cells labelling Tyrosine Hydroxylase with ab75875 at 1/10 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary midbrain neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/50 dilution (2.18 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Tyrosine Hydroxylase with purified ab75875 at 1/20 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)
    Anti-Tyrosine Hydroxylase antibody [EP1533Y] (ab75875)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (32)

Publishing research using ab75875? Please let us know so that we can cite the reference in this datasheet.

ab75875 has been referenced in 32 publications.

  • Ma J  et al. EphA1 Activation Induces Neuropathological Changes in a Mouse Model of Parkinson's Disease Through the CXCL12/CXCR4 Signaling Pathway. Mol Neurobiol 58:913-925 (2021). PubMed: 33057926
  • Bhattarai Y  et al. Role of gut microbiota in regulating gastrointestinal dysfunction and motor symptoms in a mouse model of Parkinson's disease. Gut Microbes 13:1866974 (2021). PubMed: 33459114
  • Henriques F  et al. Single-Cell RNA Profiling Reveals Adipocyte to Macrophage Signaling Sufficient to Enhance Thermogenesis. Cell Rep 32:107998 (2020). PubMed: 32755590
  • Faivre F  et al. Ablation of the tail of the ventral tegmental area compensates symptoms in an experimental model of Parkinson's disease. Neurobiol Dis 139:104818 (2020). PubMed: 32087289
  • Noonong K  et al. Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP+-Induced Mouse and Cellular Models of Parkinson's Disease. Front Neurosci 14:575459 (2020). PubMed: 33408606
View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium citrate, 0.05% Tween 20, pH 6.0
Permeabilization
Yes - 0.3% triton X100 in PBS
Specification
Brain
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Nothobranchius furzeri Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.00
Permeabilization
Yes - Triton X-100 0.3% in PBS
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Adele Leggieri

Verified customer

Submitted Aug 10 2018

Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skeletal muscle)
Permeabilization
No
Specification
skeletal muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jul 20 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Average
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 26 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Myocardium)
Specification
Myocardium
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 04 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 01 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [EP1533Y]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

Submitted Nov 01 2013

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