Overview

  • Product name

    Anti-Tyrosine Hydroxylase antibody - Neuronal Marker
    See all Tyrosine Hydroxylase primary antibodies
  • Description

    Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Pig
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Tyrosine Hydroxylase aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41527)

  • Positive control

    • ab41528 gave a positive result in the following tissue lysates: Mouse Substantia Nigra, Rat Substantia Nigra, Mouse Dopaminergic Nucleus and Rat Dopaminergic Nucleus. This antibody gave a positive result in IHC in the following FFPE tissue: Rat 6 week brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab41528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Tyrosine Hydroxylase peptide (ab41527).
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr 1/200 - 1/400.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Function

    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity

    Mainly expressed in the brain and adrenal glands.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • IHC image of Tyrosine Hydroxylase staining in Rat 6 week brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab41528 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41528, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab41528) at 1 µg/ml

    Lane 1 : Mouse Substantia Nigra at 5 µg
    Lane 2 : Rat Substantia Nigra at 10 µg
    Lane 3 : Mouse Dopaminergic Nucleus at 10 µg
    Lane 4 : Rat Dopaminergic Nucleus at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
    Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and

References

This product has been referenced in:

  • Zhang Y  et al. Procyanidin protects against 6-hydroxydopamine-induced dopaminergic neuron damage via the regulation of the PI3K/Akt signalling pathway. Biomed Pharmacother 114:108789 (2019). Read more (PubMed: 30925459) »
  • Chen C  et al. Naringenin Produces Neuroprotection Against LPS-Induced Dopamine Neurotoxicity via the Inhibition of Microglial NLRP3 Inflammasome Activation. Front Immunol 10:936 (2019). WB, IHC-FoFr ; Rat . Read more (PubMed: 31118933) »
See all 11 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-5 of 5 Abreviews

Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 13 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Heart muscle)
Specification
Heart muscle
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Nov 04 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney and Adrenal gland)
Specification
Kidney and Adrenal gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Aug 02 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%

Mr. Carl Hobbs

Verified customer

Submitted Aug 02 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Jul 09 2008

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