Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody [TH-100]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain, substantia nigra pars compacta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Brain, substantia nigra pars compacta
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: 594 anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
TH+ cells of the rat SNc. ab129991 used at 1/500 dilution.

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Submitted Feb 12 2019

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