Anti-Tyrosine Hydroxylase (phospho S19) antibody (ab51194)
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Overview
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Product nameAnti-Tyrosine Hydroxylase (phospho S19) antibody
See all Tyrosine Hydroxylase primary antibodies -
DescriptionRabbit polyclonal to Tyrosine Hydroxylase (phospho S19)
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Host speciesRabbit
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SpecificityTyrosine Hydroxylase (phospho-Ser19) antibody detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at serine19.
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Tested applicationsSuitable for: IHC-P, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthesized phosphopeptide derived from Tyrosine Hydroxylase around the phosphorylation site of Serine 19 (AVSPEQ) (Human)
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Positive control
- Human brain tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. |
Target
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FunctionPlays an important role in the physiology of adrenergic neurons.
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Tissue specificityMainly expressed in the brain and adrenal glands.
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PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
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Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Information by UniProt
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Database links
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
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Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S19) antibody (ab51194)Immunohistochemical analysis of paraffin-embedded human brain tissue using ab51194 (1/50 dilution). Left: without immunizing peptide, Right: with immunizing peptide
Protocols
Datasheets and documents
References
ab51194 has not yet been referenced specifically in any publications.
