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Rabbit polyclonal to Tyrosyl tRNA synthetase
Predicted to work with:
Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 307-528 of Human Tyrosyl tRNA synthetase (Uniprot ID: P54577).
293T, A431, H1299, HeLaS3, MOLT4, Raji whole cell lysates; A431 cells
Keep as a concentrated solution.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
1/100 - 1/1000.
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Involvement in disease
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain.
Information by UniProt
Tyrosine tRNA ligase, cytoplasmic antibody
Western blot - Anti-Tyrosyl tRNA synthetase antibody (ab155504)
Anti-Tyrosyl tRNA synthetase antibody (ab155504) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 59 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosyl tRNA synthetase antibody (ab155504)
Immunofluorescent analysis of methanol-fixed A431 cells labeling Tyrosyl tRNA synthetase with ab155504 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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