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Rabbit monoclonal [EPR9927] to Tyrosyl tRNA synthetase
ICC/IF more details Unsuitable for:
Flow Cyt,IHC-P or IP
Mouse, Rat, Human
A synthetic peptide corresponding to residues within Human Tyrosyl tRNA synthetase (P54577).
C6; PC12; Raji; HeLa and ECV 304 cell lysates; HeLa cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
1/250 - 1/500.
Is unsuitable for Flow Cyt,IHC-P or IP.
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Involvement in disease
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain.
Information by UniProt
Tyrosine tRNA ligase, cytoplasmic antibody
Western blot - Anti-Tyrosyl tRNA synthetase antibody [EPR9927] (ab154819)
All lanes : Anti-Tyrosyl tRNA synthetase antibody [EPR9927] (ab154819) at 1/1000 dilution Lane 1 : C6 cell lysate Lane 2 : PC12 cell lysate Lane 3 : Raji cell lysate Lane 4 : HeLa cell lysate Lane 5 : ECV-304 cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 59 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosyl tRNA synthetase antibody [EPR9927] (ab154819)
Immunofluorescence analysis of HeLa cells, labeling Tyrosyl tRNA synthetase using ab154819 at 1/250 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"