Recombinant
RabMAb

Recombinant Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (ab150429)

Overview

  • Product name

    Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)]
    See all Tyrosyl tRNA synthetase/TyrRS primary antibodies
  • Description

    Rabbit monoclonal [EPR9926(B)] to Tyrosyl tRNA synthetase/TyrRS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Tyrosyl tRNA synthetase/TyrRS aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human Tyrosyl tRNA synthetase/TyrRS protein (ab107141) can be used as a positive control in WB. HeLa, ECV-304, Jurkat and SH-SY5Y cell lysates; Human colon and kidney tissues.
  • General notes

     

     

    Previously labelled as Tyrosyl tRNA synthetase

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
    • Involvement in disease

      Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
    • Sequence similarities

      Belongs to the class-I aminoacyl-tRNA synthetase family.
      Contains 1 tRNA-binding domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • CMTDIC antibody
      • SYYC_HUMAN antibody
      • Tyrosine tRNA ligase, cytoplasmic antibody
      • Tyrosine tRNA ligase 1, cytoplasmic antibody
      • Tyrosyl tRNA synthetase antibody
      • Tyrosyl--tRNA ligase antibody
      • Tyrosyl-tRNA synthetase, cytoplasmic antibody
      • TyrRS antibody
      • yars antibody
      • YRS antibody
      • YTS antibody
      see all

    Images

    • All lanes : Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (ab150429) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : ECV-304 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 59 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tyrosyl tRNA synthetase/TyrRS with ab150429 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Tyrosyl tRNA synthetase/TyrRS with ab150429 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab150429 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab150429 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150429 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab150429 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab150429 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Bos taurus Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Julie Kim

    Verified customer

    Submitted May 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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