Recombinant Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (ab150429)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9926(B)] to Tyrosyl tRNA synthetase/TyrRS
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)]
See all Tyrosyl tRNA synthetase/TyrRS primary antibodies -
Description
Rabbit monoclonal [EPR9926(B)] to Tyrosyl tRNA synthetase/TyrRS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Tyrosyl tRNA synthetase/TyrRS aa 1-100. The exact sequence is proprietary.
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Positive control
- Recombinant Human Tyrosyl tRNA synthetase/TyrRS protein (ab107141) can be used as a positive control in WB. HeLa, ECV-304, Jurkat and SH-SY5Y cell lysates; Human colon and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9926(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
Target
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Function
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). -
Involvement in disease
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8565 Human
- Entrez Gene: 107271 Mouse
- Entrez Gene: 313047 Rat
- Omim: 603623 Human
- SwissProt: P54577 Human
- SwissProt: Q91WQ3 Mouse
- SwissProt: Q4KM49 Rat
- Unigene: 213264 Human
see all -
Alternative names
- CMTDIC antibody
- SYYC_HUMAN antibody
- Tyrosine tRNA ligase, cytoplasmic antibody
see all
Images
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All lanes : Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (ab150429) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : ECV-304 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 59 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tyrosyl tRNA synthetase/TyrRS with ab150429 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Tyrosyl tRNA synthetase/TyrRS with ab150429 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab150429 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab150429 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150429 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab150429 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab150429 has been referenced in 4 publications.
- Huh D et al. A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. EMBO J 40:e106696 (2021). PubMed: 33346941
- Zhang C et al. YARS as an oncogenic protein that promotes gastric cancer progression through activating PI3K-Akt signaling. J Cancer Res Clin Oncol 146:329-342 (2020). PubMed: 31912229
- Sleigh JN et al. Altered Sensory Neuron Development in CMT2D Mice Is Site-Specific and Linked to Increased GlyRS Levels. Front Cell Neurosci 14:232 (2020). PubMed: 32848623
- Huang S et al. Discovery of human TyrRS inhibitors by structure-based virtual screening, structural optimization, and bioassays. RSC Adv 9:9323-9330 (2019). PubMed: 35517706