Key features and details
- Rabbit polyclonal to Tyrosyl tRNA synthetase/TyrRS - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Tyrosyl tRNA synthetase/TyrRS antibody - N-terminal
See all Tyrosyl tRNA synthetase/TyrRS primary antibodies
DescriptionRabbit polyclonal to Tyrosyl tRNA synthetase/TyrRS - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human Tyrosyl tRNA synthetase/TyrRS aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: P54577
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Previously labelled as Tyrosyl tRNA synthetase
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226398 was affinity purified using an epitope specific to Tyrosyl-tRNA Synthetase immobilized on solid support.
Our Abpromise guarantee covers the use of ab226398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 59 kDa.|
FunctionCatalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Involvement in diseaseDefects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain.
- Information by UniProt
- CMTDIC antibody
- SYYC_HUMAN antibody
- Tyrosine tRNA ligase, cytoplasmic antibody
All lanes : Anti-Tyrosyl tRNA synthetase/TyrRS antibody - N-terminal (ab226398) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Tyrosyl tRNA synthetase/TyrRS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226398 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226398 at 0.4 µg/ml.
Lane 1: ab226398 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226398 has not yet been referenced specifically in any publications.