Product nameAnti-Tyrosyl tRNA synthetase/TyrRS antibody - N-terminal
See all Tyrosyl tRNA synthetase/TyrRS primary antibodies
DescriptionRabbit polyclonal to Tyrosyl tRNA synthetase/TyrRS - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Orangutan, Xenopus tropicalis
Synthetic peptide within Human Tyrosyl tRNA synthetase/TyrRS aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: P54577
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Previously labelled as Tyrosyl tRNA synthetase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226398 was affinity purified using an epitope specific to Tyrosyl-tRNA Synthetase immobilized on solid support.
Our Abpromise guarantee covers the use of ab226398 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 59 kDa.|
FunctionCatalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Involvement in diseaseDefects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain.
- Information by UniProt
- CMTDIC antibody
- SYYC_HUMAN antibody
- Tyrosine tRNA ligase, cytoplasmic antibody
All lanes : Anti-Tyrosyl tRNA synthetase/TyrRS antibody - N-terminal (ab226398) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Tyrosyl tRNA synthetase/TyrRS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226398 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226398 at 0.4 µg/ml.
Lane 1: ab226398 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab226398 has not yet been referenced specifically in any publications.